Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215257_0brw CAAGTCAGAATTCCTGGTAGGGGCGGTTTCAG(+ACTCAC)GATCCCACAGAAATGAAGGGACACAGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240604215256_m4tn TGGGAGAAGACTGTGTCCCTTCATTTCTGTGG(+ACTCAC)GATCCTGAAACCGCCCCTACCAGGAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240604215253_uqnm CATCCAGGCCCCCAATCTTTTATATTGAAGGG(+ACTCAC)GATCCAACTTCCAGGATGAAAGGCAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240604215252_ks7z ACCAGGCTCTCTGCCTTTCATCCTGGAAGTTG(+ACTCAC)GATCCCCTTCAATATAAAAGATTGGGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240604215250_98d8 GCTGAGCAAGAAAGGCTGTCTGAGCCATGGTG(+ACTCAC)GATCCCCAGGGAGGGAATGTCCCCTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240604215248_onwv GGGCAGGGGGGAGGGGACATTCCCTCCCTGGG(+ACTCAC)GATCCACCATGGCTCAGACAGCCTTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240604215246_3qcj GCCTACTTCTGGATCACCCTCATCCAGTGCTG(+ACTCAC)GTACCCCACAGCACTAAATCCAGGAACCCTG NGG 10 8 17 8 24 0 100 5 10
    240604215244_tk57 AAGCCCAGGGTTCCTGGATTTAGTGCTGTGGG(+ACTCAC)GTACCAGCACTGGATGAGGGTGATCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604215243_1dgf GGAAGCAGAAGGCAGAGCTGGCCCTGGTACTG(+ACTCAC)GTACCCCACCCTTCAGCGTAGCCCTTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240604215241_3ee6 AGCTGTCACCAAGGGCTACGCTGAAGGGTGGG(+ACTCAC)GTACCAGTACCAGGGCCAGCTCTGCCTTCTG NGG 10 8 17 8 24 0 100 5 10