Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215409_o7in CCAACTTCAGCCCATTCACGCCCCTGCCTTTG(+ACTCAC)GTACCAGCTACTTTCTCCACCCACCCAACTC NGG 10 8 17 8 24 0 100 5 10
    240604215408_4bwy AAGGGGAGTTGGGTGGGTGGAGAAAGTAGCTG(+ACTCAC)GTACCAAAGGCAGGGGCGTGAATGGGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240604215406_pwzd AGATTGGGGCAGTGGGACAAGCTATAATCCTG(+ACTCAC)GTACCCAGGAGGCAGTGGTATGGAGATTTTG NGG 10 8 17 8 24 0 100 5 10
    240604215404_x9v1 GACCACAAAATCTCCATACCACTGCCTCCTGG(+ACTCAC)GTACCAGGATTATAGCTTGTCCCACTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240604215402_gf4o TGCAAAACAAAACAAAACAAAACAAAACAAAG(+ACTCAC)GATCCTGTATTGGCAGAGATGGTGGTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604215401_q056 ATGCATCTTTACCACCATCTCTGCCAATACAG(+ACTCAC)GATCCTTTGTTTTGTTTTGTTTTGTTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240604215359_47ra CCAGTTGTAATTGCTTTGAACCAAGCACCTCG(+ACTCAC)GATCCTGACTACTTTAAACACTGAGCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240604215357_3ktr TACCACTGGAGCTCAGTGTTTAAAGTAGTCAG(+ACTCAC)GATCCGAGGTGCTTGGTTCAAAGCAATTACA NGG 10 8 17 8 24 0 100 5 10
    240604215356_xjha GCATGTATGTCTGTGTTTCAGTAGCATGCTTG(+ACTCAC)GTACCCAAGGAAGCCAGAAAAAGGCATTAGA NGG 10 8 17 8 24 0 100 5 10
    240604215354_0o86 GGGCATCTAATGCCTTTTTCTGGCTTCCTTGG(+ACTCAC)GTACCAAGCATGCTACTGAAACACAGACATA NGG 10 8 17 8 24 0 100 5 10