Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215518_7jxg TCCTCCACAAATAAACGTAATTAAATCTAAAG(+ACTCAC)GTACCCTGAGATTTCTGTATAGGAAACAACT NGG 10 8 17 8 24 0 100 5 10
    240604215516_w660 AAAACAGTTGTTTCCTATACAGAAATCTCAGG(+ACTCAC)GTACCTTTAGATTTAATTACGTTTATTTGTG NGG 10 8 17 8 24 0 100 5 10
    240604215515_1h93 AGGTTTCTAGAAACACGCAGACACTGAGAATG(+ACTCAC)GTACCCAGGGTCCTGCTCTTTACCACAGGTT NGG 10 8 17 8 24 0 100 5 10
    240604215512_mmux GCATGAACCTGTGGTAAAGAGCAGGACCCTGG(+ACTCAC)GTACCATTCTCAGTGTCTGCGTGTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240604215510_3ija AGAGGTTAGATGTTCTTGGCCTGCAGGGACAG(+ACTCAC)GTACCTATAGTGTTGGCATAGTCAGGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240604215509_49h2 TGCTGGGAAACCTGACTATGCCAACACTATAG(+ACTCAC)GTACCTGTCCCTGCAGGCCAAGAACATCTAA NGG 10 8 17 8 24 0 100 5 10
    240604215508_l6hx TGCCGTCGGGCTGGATCCTAGGTGGACACTTG(+ACTCAC)GTACCTCTCTGTTGCTGTTTCTTGGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604215507_zrnu AGCTGGGCTCTCCAAGAAACAGCAACAGAGAG(+ACTCAC)GTACCAAGTGTCCACCTAGGATCCAGCCCGA NGG 10 8 17 8 24 0 100 5 10
    240604215505_6ee4 CCCTAAGGAGACGTCAGGGTGCCGTCGGGCTG(+ACTCAC)GATCCTAGGTGGACACTTGGTACCTCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240604215503_4403 CAGCAACAGAGAGGTACCAAGTGTCCACCTAG(+ACTCAC)GATCCAGCCCGACGGCACCCTGACGTCTCCT NGG 10 8 17 8 24 0 100 5 10