Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215607_a20a TTGTAAGAAGCATTTTAAGCAGCTGCTGTAGG(+ACTCAC)GTACCTTAAAAAAAAAACTTCCAGTAGAGTA NGG 10 8 17 8 24 0 100 5 10
    240604215605_50tj ATTTATACTCTACTGGAAGTTTTTTTTTTAAG(+ACTCAC)GTACCCTACAGCAGCTGCTTAAAATGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604215602_yf92 CTCGAGAAACGCAGTCTCGGCCTCCTCCCAGG(+ACTCAC)GATCCAAACGCCAGCCACCCGCCCGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240604215601_wftj TCTCTAAAAGCCGGGCGGGTGGCTGGCGTTTG(+ACTCAC)GATCCCTGGGAGGAGGCCGAGACTGCGTTTC NGG 10 8 17 8 24 0 100 5 10
    240604215559_g2gv GACTGTGAATGAAGAGCTGTGCTGTGTGGGTG(+ACTCAC)GTACCTGCTGTCTCCTATCTGCTGGGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240604215558_ad4c AGATCTAAGACCCAGCAGATAGGAGACAGCAG(+ACTCAC)GTACCACCCACACAGCACAGCTCTTCATTCA NGG 10 8 17 8 24 0 100 5 10
    240604215557_z7af GATGTTGCTGCTGTTAGGAAGGTAGGCAGAAG(+ACTCAC)GATCCATGTCTGTGCCGAGAGGTGTGACGCC NGG 10 8 17 8 24 0 100 5 10
    240604215556_448j CCCCAGGCGTCACACCTCTCGGCACAGACATG(+ACTCAC)GATCCTTCTGCCTACCTTCCTAACAGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604215555_x5iz CTCCTGTCGGGTATTTCTTACTGGCACAGTAG(+ACTCAC)GTACCTGGATGTTCACAGAGCATCCGCCCCG NGG 10 8 17 8 24 0 100 5 10
    240604215553_zf1a TGGGACGGGGCGGATGCTCTGTGAACATCCAG(+ACTCAC)GTACCTACTGTGCCAGTAAGAAATACCCGAC NGG 10 8 17 8 24 0 100 5 10