Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215645_7fmn CCCATCCTGTCAGGTTGGTGCGGAAGGGCTAG(+ACTCAC)GATCCTTGCCGCCCTTCCCTTCGGTGCCGGA NGG 10 8 17 8 24 0 100 5 10
    240604215643_q8ov AGGGTTCCGGCACCGAAGGGAAGGGCGGCAAG(+ACTCAC)GATCCTAGCCCTTCCGCACCAACCTGACAGG NGG 10 8 17 8 24 0 100 5 10
    240604215642_x89w AGGCAGGTTCTCTCTGCTGTCCTCAGGCAGTG(+ACTCAC)GTACCCTTGGGGGTCTCCTTGGTCTTCACAC NGG 10 8 17 8 24 0 100 5 10
    240604215640_a0sa CGTGTGTGTGAAGACCAAGGAGACCCCCAAGG(+ACTCAC)GTACCACTGCCTGAGGACAGCAGAGAGAACC NGG 10 8 17 8 24 0 100 5 10
    240604215638_zvke GCCACGGCTTGCGATGTATAAGCACACAGAAG(+ACTCAC)GATCCAGTGCAATAAAGACGTGAAACGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604215637_p5f8 ACACACACACGTTTCACGTCTTTATTGCACTG(+ACTCAC)GATCCTTCTGTGTGCTTATACATCGCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240604215634_uhsw TGAAACCACTCAAAGTCACAAAGAAACCCAGG(+ACTCAC)GATCCCCTAAGCTGTGGTCCAGGTGCCCTAG NGG 10 8 17 8 24 0 100 5 10
    240604215632_thn5 AAGTACTAGGGCACCTGGACCACAGCTTAGGG(+ACTCAC)GATCCCTGGGTTTCTTTGTGACTTTGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604215630_fra7 CACTGGAGGTGCAGGTGCCTGGGCCAGCCTTG(+ACTCAC)GATCCATATCCAGTATTTGGCAGATTCATGC NGG 10 8 17 8 24 0 100 5 10
    240604215629_vyil GGCAGGCATGAATCTGCCAAATACTGGATATG(+ACTCAC)GATCCAAGGCTGGCCCAGGCACCTGCACCTC NGG 10 8 17 8 24 0 100 5 10