Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215739_s7ln GCTCATAATTATCTGTAACTCCAATTTCAGGG(+ACTCAC)GATCCAACACCCTCATAAAGACACACATGCA NGG 10 8 17 8 24 0 100 5 10
    240604215737_1agz TTGCCTGCATGTGTGTCTTTATGAGGGTGTTG(+ACTCAC)GATCCCCTGAAATTGGAGTTACAGATAATTA NGG 10 8 17 8 24 0 100 5 10
    240604215736_9uux AGTGTCTGGGTTTGGTGGTTGTTTATGGGATG(+ACTCAC)GATCCCCAGGTGGGGCAGTCTTTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604215733_9voe GGAAGGACCATCCAAAGACTGCCCCACCTGGG(+ACTCAC)GATCCATCCCATAAACAACCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240604215731_w6a8 GGATTAAGGTACTATGCCAAGCGTTTAAAGTG(+ACTCAC)GATCCTGTGGATAAACTTAGCTTCAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240604215729_wqlc ACCTGTGTTCTTGAAGCTAAGTTTATCCACAG(+ACTCAC)GATCCACTTTAAACGCTTGGCATAGTACCTT NGG 10 8 17 8 24 0 100 5 10
    240604215728_b24u TGAGGTGTTCTGGAGGGAATGGGCATGACAGG(+ACTCAC)GTACCTTAGGCTGAAAGCCAGCAAAGGCACA NGG 10 8 17 8 24 0 100 5 10
    240604215726_dppf CATCCTGTGCCTTTGCTGGCTTTCAGCCTAAG(+ACTCAC)GTACCCTGTCATGCCCATTCCCTCCAGAACA NGG 10 8 17 8 24 0 100 5 10
    240604215723_6ycr TGGAGGCCCCAGGGCCAGAGTCCAAAGCCAGG(+ACTCAC)GTACCAGCTGCAGGAGATGGAGAAGGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240604215722_0myc CTCCCTGGCCCCTTCTCCATCTCCTGCAGCTG(+ACTCAC)GTACCCTGGCTTTGGACTCTGGCCCTGGGGC NGG 10 8 17 8 24 0 100 5 10