Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215850_8lhq GTAAAAAAAAAAAAAAAGGCAGATGGCAGATG(+ACTCAC)GATCCTGGAGCTTTTTCTCTCTTTTTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240604215848_uv4s CTGGGGAAAAAAAAAGAGAGAAAAAGCTCCAG(+ACTCAC)GATCCATCTGCCATCTGCCTTTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604215846_swb0 CGAAGAAAAGTGCCTCTGTGTACTCTCCCTCG(+ACTCAC)GATCCAGTTTTACCCTCAGCTGCATGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240604215845_3yqh CAAACGTTTTCATGCAGCTGAGGGTAAAACTG(+ACTCAC)GATCCGAGGGAGAGTACACAGAGGCACTTTT NGG 10 8 17 8 24 0 100 5 10
    240604215842_qei8 AGTGTGAGCAACCACTTCTGTGTTTACCTGGG(+ACTCAC)GATCCATCCCACAATCAGCCTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240604215840_yits GGTGTCAGCGTTTGGAGGCTGATTGTGGGATG(+ACTCAC)GATCCCCAGGTAAACACAGAAGTGGTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240604215837_9wir GGATGGACCATCCAGAGACTGCCCCACCCGGG(+ACTCAC)GATCCATCCCATAATCAGCCACCAAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240604215836_pztq AGTGTCTGCATTTGGTGGCTGATTATGGGATG(+ACTCAC)GATCCCCGGGTGGGGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604215834_kceu CAGGGCCAAGAAGTAGGAGTGGGTGAGTAGGG(+ACTCAC)GATCCGGGTGGAGGTATAGGGGACTTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240604215832_yszd GCTATCCTGAAAGTCCCCTATACCTCCACCCG(+ACTCAC)GATCCCCTACTCACCCACTCCTACTTCTTGG NGG 10 8 17 8 24 0 100 5 10