Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215921_9rwg ATAGCATCCTGGTGAAATGAGCTATGTCTCAG(+ACTCAC)GTACCACTAAACATCAGCCACTCACCTTTAG NGG 10 8 17 8 24 0 100 5 10
    240604215920_g5y7 CTGGCCTAAAGGTGAGTGGCTGATGTTTAGTG(+ACTCAC)GTACCTGAGACATAGCTCATTTCACCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240604215918_7m3u CTTTATGGTCAAGAGATTATTATGACACAGGG(+ACTCAC)GTACCATTAGCGTAACAGCCCTCCAGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240604215917_nqat TGAGCACTCTCTGGAGGGCTGTTACGCTAATG(+ACTCAC)GTACCCCTGTGTCATAATAATCTCTTGACCA NGG 10 8 17 8 24 0 100 5 10
    240604215915_usvt GGCACATGGGTCAGAACAAAGTTGCATTATGG(+ACTCAC)GTACCAGCGATCCAATTAATTAATATTTATA NGG 10 8 17 8 24 0 100 5 10
    240604215914_ntpb CCAGCTATAAATATTAATTAATTGGATCGCTG(+ACTCAC)GTACCCATAATGCAACTTTGTTCTGACCCAT NGG 10 8 17 8 24 0 100 5 10
    240604215912_ulq6 GTCATGGGCACTGTGGCTGAAGTGGCTGCTTG(+ACTCAC)GTACCAGCCGTCTCAGTAGCAGGAGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604215911_r4wz CTTGTGGCTCACTCCTGCTACTGAGACGGCTG(+ACTCAC)GTACCAAGCAGCCACTTCAGCCACAGTGCCC NGG 10 8 17 8 24 0 100 5 10
    240604215909_zrcp AACATTCCAGGCCGTTCGGAAGGCATCACTGG(+ACTCAC)GTACCAGACTTGAAACCAGTCTTCAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240604215908_xm6o ACACTTGTTACTGAAGACTGGTTTCAAGTCTG(+ACTCAC)GTACCCAGTGATGCCTTCCGAACGGCCTGGA NGG 10 8 17 8 24 0 100 5 10