Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215831_6cr2 GAGGTTGTCTCTGGCTCTGTTGTCTGCTGTTG(+ACTCAC)GATCCCTTTCTCCTACCTGGACTGTCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240604215829_1dlu GGCCCAACCAGACAGTCCAGGTAGGAGAAAGG(+ACTCAC)GATCCAACAGCAGACAACAGAGCCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240604215827_owpa GGAAGAAGAACCTTTGTAACCTGTTAATGGAG(+ACTCAC)GATCCTTATAAAACAGAACAACTCTGTATAA NGG 10 8 17 8 24 0 100 5 10
    240604215826_2qjl CCGGTTTATACAGAGTTGTTCTGTTTTATAAG(+ACTCAC)GATCCTCCATTAACAGGTTACAAAGGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240604215825_vxsf TGGGACACGCCCCCCCCCCCCCACTTGAGCTG(+ACTCAC)GATCCCACTTTGTGCCTTTCTCTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240604215823_7a00 GAAAAGAAGGTCCAGAGAAAGGCACAAAGTGG(+ACTCAC)GATCCAGCTCAAGTGGGGGGGGGGGGGCGTG NGG 10 8 17 8 24 0 100 5 10
    240604215822_yutx ATGGTAATAAATAAATAAGATTTCTTTTAATG(+ACTCAC)GATCCAGACTTACCTGAGGACAACTGTAGGG NGG 10 8 17 8 24 0 100 5 10
    240604215821_rau3 ATTAACCCTACAGTTGTCCTCAGGTAAGTCTG(+ACTCAC)GATCCATTAAAAGAAATCTTATTTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240604215818_yztw GATCAGCATCCAGGCCCATCCATCAGCCTTCG(+ACTCAC)GATCCTGCTCTGCTCTTTATTCTGCTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240604215817_vt3k GGAGTGAGACAGCAGAATAAAGAGCAGAGCAG(+ACTCAC)GATCCGAAGGCTGATGGATGGGCCTGGATGC NGG 10 8 17 8 24 0 100 5 10