Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215816_2myk AACCTCTGGGTTTGGTGGCTGATTATGGGATG(+ACTCAC)GATCCCCAGGTGGGGTAGTCTCTGGATAGTC NGG 10 8 17 8 24 0 100 5 10
    240604215813_8560 GGATGGACTATCCAGAGACTACCCCACCTGGG(+ACTCAC)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240604215811_4v6v AATAGAAAAAAAAGTAAAACCTCACTTCACAG(+ACTCAC)GATCCCCCTCCATTCCCTATGTTCCTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240604215809_u7wk ACACCTTTACAGGAACATAGGGAATGGAGGGG(+ACTCAC)GATCCTGTGAAGTGAGGTTTTACTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604215807_jjjo GTGCTTTGGTTGTATGATGGGGGAGTGGGCAG(+ACTCAC)GTACCCACTTGCTGCCTCTGTCCTCATCTAT NGG 10 8 17 8 24 0 100 5 10
    240604215805_2b8i TCTGGATAGATGAGGACAGAGGCAGCAAGTGG(+ACTCAC)GTACCTGCCCACTCCCCCATCATACAACCAA NGG 10 8 17 8 24 0 100 5 10
    240604215803_9kmo CATCCCTTTGTCACTAAAGGGAACAACATGGG(+ACTCAC)GTACCAGGCAGTCAAAAGCTGTAAAAACCCC NGG 10 8 17 8 24 0 100 5 10
    240604215801_yaj6 AAAATGGGGTTTTTACAGCTTTTGACTGCCTG(+ACTCAC)GTACCCCATGTTGTTCCCTTTAGTGACAAAG NGG 10 8 17 8 24 0 100 5 10
    240604215759_rmf1 CATCCCTTTGTCACTAAAGGGAACAACATGGG(+ACTCAC)GTACCAGGCAGTCAAAAGCTGTAAAAACCCC NGG 10 8 17 8 24 0 100 5 10
    240604215757_hagf AAAATGGGGTTTTTACAGCTTTTGACTGCCTG(+ACTCAC)GTACCCCATGTTGTTCCCTTTAGTGACAAAG NGG 10 8 17 8 24 0 100 5 10