Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215907_zroz CCGTGGGATTATAGGAAAACCAGGGCCTCTTG(+ACTCAC)GTACCCTGTCCTCAGAGTGTGTGACAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240604215905_kgtt TTACAGTGGCTGTCACACACTCTGAGGACAGG(+ACTCAC)GTACCAAGAGGCCCTGGTTTTCCTATAATCC NGG 10 8 17 8 24 0 100 5 10
    240604215904_u7sb GCACCTCCGAAGCTCTTAGTAATGTTGTGCTG(+ACTCAC)GATCCTGTAAGTTCGCTCTGCAGTCACTCGC NGG 10 8 17 8 24 0 100 5 10
    240604215903_e3tb GGTTTGCGAGTGACTGCAGAGCGAACTTACAG(+ACTCAC)GATCCAGCACAACATTACTAAGAGCTTCGGA NGG 10 8 17 8 24 0 100 5 10
    240604215901_w2gf TGATGCTCCACAAACTGTCTCATTAATCCATG(+ACTCAC)GATCCTAAAACAAATTCTCTTTGTGTATTGT NGG 10 8 17 8 24 0 100 5 10
    240604215859_yh7q CACACACAATACACAAAGAGAATTTGTTTTAG(+ACTCAC)GATCCATGGATTAATGAGACAGTTTGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240604215857_luk8 TCTTCCTTTTGAAGCTAGGCTGTGCTGTGTGG(+ACTCAC)GATCCAGTGGTCTGTTCTTTACTGGAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240604215855_mymj AACACATACCTCCAGTAAAGAACAGACCACTG(+ACTCAC)GATCCCACACAGCACAGCCTAGCTTCAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604215854_6byl AGTAAAACCTTTAAACATGGTGACTATCTTCG(+ACTCAC)GATCCCAAGGAGAATATTAACAGGCACCAGG NGG 10 8 17 8 24 0 100 5 10
    240604215852_yfoc GAGTTCCTGGTGCCTGTTAATATTCTCCTTGG(+ACTCAC)GATCCGAAGATAGTCACCATGTTTAAAGGTT NGG 10 8 17 8 24 0 100 5 10