Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215756_cjc7 TCTCTAGCCAGCCCTGCTGCTCCTGTTGTCTG(+ACTCAC)GTACCCAGGTGACTGAGGACATGACTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240604215753_g8wn TCCTTCCACCAGTCATGTCCTCAGTCACCTGG(+ACTCAC)GTACCAGACAACAGGAGCAGCAGGGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240604215751_yh7u GTGCACAGTTCTGTGAGGCTTTGGGGCAAGGG(+ACTCAC)GTACCAAGAGCCCAAGAGAGCCTTTCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240604215750_hwed TTTAGAACAAGAAAGGCTCTCTTGGGCTCTTG(+ACTCAC)GTACCCCTTGCCCCAAAGCCTCACAGAACTG NGG 10 8 17 8 24 0 100 5 10
    240604215749_u9jn TAGGGAGGGATGTCAAGGCCAAGAGTCATACG(+ACTCAC)GTACCCAGTAACTGCTGGTGTAGCTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240604215747_t9jz TGCCTCCCAAGAGCTACACCAGCAGTTACTGG(+ACTCAC)GTACCGTATGACTCTTGGCCTTGACATCCCT NGG 10 8 17 8 24 0 100 5 10
    240604215745_w7dt CCAACAGGGAGGGACCAAGGGCAGCCACCAGG(+ACTCAC)GATCCTATCTCACCTCCGATGCCTGCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240604215744_tchn CCTGGACACAGCAGGCATCGGAGGTGAGATAG(+ACTCAC)GATCCCTGGTGGCTGCCCTTGGTCCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240604215742_hrjp CATGGGCTAGTTTCCATTAGGCCCTCCATGGG(+ACTCAC)GATCCTAAAGAGGGAGAGGGAATGAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604215741_oxwa CTGCTTTTCTCTCATTCCCTCTCCCTCTTTAG(+ACTCAC)GATCCCCATGGAGGGCCTAATGGAAACTAGC NGG 10 8 17 8 24 0 100 5 10