Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215445_xu9e CCTGAACCCATGGAACTTCATGACTTTTGCTG(+ACTCAC)GATCCATTTCCCTTAGTGGCTTCCAGAGCCC NGG 10 8 17 8 24 0 100 5 10
    240604215444_g96l AACTGGGGCTCTGGAAGCCACTAAGGGAAATG(+ACTCAC)GATCCAGCAAAAGTCATGAAGTTCCATGGGT NGG 10 8 17 8 24 0 100 5 10
    240604215442_7era CTGGGTGTGGCCTCAGCTCCTGTGCTTGTGTG(+ACTCAC)GATCCCTAAAGGGTGTGCTAAGGAGCAATTG NGG 10 8 17 8 24 0 100 5 10
    240604215441_pyyi CAGTGCAATTGCTCCTTAGCACACCCTTTAGG(+ACTCAC)GATCCACACAAGCACAGGAGCTGAGGCCACA NGG 10 8 17 8 24 0 100 5 10
    240604215439_8bs4 CAGTTTGCGGACAAACCTGATGACCTGAGTTG(+ACTCAC)GATCCCTGACCATAAGGTAGAAGAGACCTGA NGG 10 8 17 8 24 0 100 5 10
    240604215437_646t AGGAATCAGGTCTCTTCTACCTTATGGTCAGG(+ACTCAC)GATCCAACTCAGGTCATCAGGTTTGTCCGCA NGG 10 8 17 8 24 0 100 5 10
    240604215436_j8tx TGGGGCGCACATCCCACCTCAGTGTTCTAAAG(+ACTCAC)GATCCAGGGCCACTTTGTCTTCAAGTGTCAT NGG 10 8 17 8 24 0 100 5 10
    240604215433_ga4v CACAAATGACACTTGAAGACAAAGTGGCCCTG(+ACTCAC)GATCCTTTAGAACACTGAGGTGGGATGTGCG NGG 10 8 17 8 24 0 100 5 10
    240604215432_oi1r AACCCTCTTGGAATCATGATTTGTATTTTATG(+ACTCAC)GTACCTTCTCTCTAAACTCATAGATTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240604215431_6i6v AACAAAAAACAATCTATGAGTTTAGAGAGAAG(+ACTCAC)GTACCATAAAATACAAATCATGATTCCAAGA NGG 10 8 17 8 24 0 100 5 10