Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215126_fyrr TGCTTCAAGCTGTGAGGAAGCCGCAGCCCAGG(+ACTCAC)GATCCCAAGCTTTTCGGGAGTGGCCTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240604215124_tfwr CCACTGAACAAGGCCACTCCCGAAAAGCTTGG(+ACTCAC)GATCCCTGGGCTGCGGCTTCCTCACAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240604215121_5vtp GCTCACAACTGTCTATAATTCCAGTTCCAGGG(+ACTCAC)GATCCAACACCCTATTCTCCTGGTTTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240604215120_bvqd ATATCTGAGGAAACCAGGAGAATAGGGTGTTG(+ACTCAC)GATCCCCTGGAACTGGAATTATAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240604215118_1wfz GTGTAGTGTTCCTAGATTCAGCTGAAGACCTG(+ACTCAC)GTACCTAGGGTTCCTAACGGGATGGTGTTAG NGG 10 8 17 8 24 0 100 5 10
    240604215115_vn56 GTTTCCTAACACCATCCCGTTAGGAACCCTAG(+ACTCAC)GTACCAGGTCTTCAGCTGAATCTAGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240604215113_bom2 GGCCTTGGGGACCCAGGGTCCTGGAGATGGAG(+ACTCAC)GTACCTTGGATTCTGAGACCTAATTAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240604215111_tz6p GAGGGCTGACTAATTAGGTCTCAGAATCCAAG(+ACTCAC)GTACCTCCATCTCCAGGACCCTGGGTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240604215108_d3t3 CTTGAAAAACAGAGGCTTTGTCGTTCTCATAG(+ACTCAC)GATCCACAGTGTCTGAGCTATCCACGCTGAC NGG 10 8 17 8 24 0 100 5 10
    240604215106_pi2u TTGGTGTCAGCGTGGATAGCTCAGACACTGTG(+ACTCAC)GATCCTATGAGAACGACAAAGCCTCTGTTTT NGG 10 8 17 8 24 0 100 5 10