Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604215028_ofdj ACTGAACCTGGAGCTGCCCAGCAGGCTTCCGG(+ACTCAC)GATCCTCCAGGGTCTGCTTCCACAGAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604215026_z7wi GACTCCTGGCTCTGTGGAAGCAGACCCTGGAG(+ACTCAC)GATCCCGGAAGCCTGCTGGGCAGCTCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240604215025_6ijr GAGTCAGTTCTTGAGCCCAGCTTTCCAGTGAG(+ACTCAC)GATCCGCACCTGCGGGATGTGTGTCCTATTC NGG 10 8 17 8 24 0 100 5 10
    240604215023_6cw5 CACATGAATAGGACACACATCCCGCAGGTGCG(+ACTCAC)GATCCTCACTGGAAAGCTGGGCTCAAGAACT NGG 10 8 17 8 24 0 100 5 10
    240604215021_pb1x AATAATGCTCACTTCCCAGTCTGTGGCCACGG(+ACTCAC)GATCCCATGTGTCTTTTTGCTTGTGGCAGAT NGG 10 8 17 8 24 0 100 5 10
    240604215019_i7me AAGAAATCTGCCACAAGCAAAAAGACACATGG(+ACTCAC)GATCCCGTGGCCACAGACTGGGAAGTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240604215017_1oib AGGGCAGGACCTGGTCCATCTCTGCGGGACTG(+ACTCAC)GTACCAACCAATAAGGCAGAGGGGGTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240604215016_q7rn GGCGAGCTCAACCCCCTCTGCCTTATTGGTTG(+ACTCAC)GTACCAGTCCCGCAGAGATGGACCAGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240604215015_tpqd GGTGTCAGCAGTTGGAGCAGCCTGGAGTTCTG(+ACTCAC)GATCCTGGCTCCTAGGTGGTTTTCACATCAG NGG 10 8 17 8 24 0 100 5 10
    240604215013_2hwn AGAGGCTGATGTGAAAACCACCTAGGAGCCAG(+ACTCAC)GATCCAGAACTCCAGGCTGCTCCAACTGCTG NGG 10 8 17 8 24 0 100 5 10