Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214937_6pfa ACGCCTTTAGTCTCCGGATGTCTCAAATTATG(+ACTCAC)GATCCTGAGCGCAGCCGTCTGCTCTTTCATG NGG 10 8 17 8 24 0 100 5 10
    240604214936_5bvk TTATGCATGAAAGAGCAGACGGCTGCGCTCAG(+ACTCAC)GATCCATAATTTGAGACATCCGGAGACTAAA NGG 10 8 17 8 24 0 100 5 10
    240604214934_l1ql CCTGTGTCTCAGGGACGCCTCTACTTTCTTGG(+ACTCAC)GATCCGGGACCGTTCTCAGAGGCACTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240604214931_s88m TTCCGTCCCCAGTGCCTCTGAGAACGGTCCCG(+ACTCAC)GATCCCAAGAAAGTAGAGGCGTCCCTGAGAC NGG 10 8 17 8 24 0 100 5 10
    240604214929_3ron CAGCAAGATGCTCCTGATGCCCTTTGGTCCAG(+ACTCAC)GATCCACTGCCTCCTTGTTCTAGAGGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240604214928_whjk GATGTCTGAGCCTCTAGAACAAGGAGGCAGTG(+ACTCAC)GATCCTGGACCAAAGGGCATCAGGAGCATCT NGG 10 8 17 8 24 0 100 5 10
    240604214926_ufhq CCGCATGATGGCTGGGTCTGAAATCGCAAATG(+ACTCAC)GATCCAGCCTGATGTCCTCGCAGTTGCACAT NGG 10 8 17 8 24 0 100 5 10
    240604214924_n8hc CCCACATGTGCAACTGCGAGGACATCAGGCTG(+ACTCAC)GATCCATTTGCGATTTCAGACCCAGCCATCA NGG 10 8 17 8 24 0 100 5 10
    240604214922_pooc ATAGATACACTGACTTGGCTTCCTGGGCTGGG(+ACTCAC)GTACCAGGAAGGAAGAAACCAGACAGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240604214921_h0lw AGGGAAAAACCTGTCTGGTTTCTTCCTTCCTG(+ACTCAC)GTACCCCAGCCCAGGAAGCCAAGTCAGTGTA NGG 10 8 17 8 24 0 100 5 10