Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214919_swwx GAGAACTCAGCTGGCTTTCTGGGTTTTCTCAG(+ACTCAC)GTACCTGTTTCCTCAGGGCTGTTGTTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240604214918_pai3 CCTCTGTGGGAACAACAGCCCTGAGGAAACAG(+ACTCAC)GTACCTGAGAAAACCCAGAAAGCCAGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240604214917_aonl GGCTGCTGGCCTCCCCTCCATCCTCCAGGACG(+ACTCAC)GATCCATGTTCATTCCTATCCATACCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240604214915_k4uu GTAGGACCAAGGTATGGATAGGAATGAACATG(+ACTCAC)GATCCGTCCTGGAGGATGGAGGGGAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604214914_d1ap TATTTGGCCAGCCCTCAACCTCCTCTAGTTTG(+ACTCAC)GATCCAAGGGCCCTGTATGTATGAGGAGTAA NGG 10 8 17 8 24 0 100 5 10
    240604214911_uzug CTACTTTACTCCTCATACATACAGGGCCCTTG(+ACTCAC)GATCCAAACTAGAGGAGGTTGAGGGCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240604214909_bfmb CCAAAAAAAAAAAAGTGCGTGGGAGGAGCCGG(+ACTCAC)GATCCTTTGGTCTCCCTCTCTGCTCCACTTT NGG 10 8 17 8 24 0 100 5 10
    240604214907_0hx3 GAGGCAAAGTGGAGCAGAGAGGGAGACCAAAG(+ACTCAC)GATCCCGGCTCCTCCCACGCACTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604214905_jt16 CCTGAGCCTCCCTCGGGCACCTGTTGCTTCTG(+ACTCAC)GATCCGAATAATCCAGGAGGCTCCCCACCCT NGG 10 8 17 8 24 0 100 5 10
    240604214904_c8rq GCCACAGGGTGGGGAGCCTCCTGGATTATTCG(+ACTCAC)GATCCAGAAGCAACAGGTGCCCGAGGGAGGC NGG 10 8 17 8 24 0 100 5 10