Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214953_6vcn TCCCGTCATGTTTCTCCTTACTCATCCAATTG(+ACTCAC)GTACCCACAACTGAAGAGCTCGTGTATTTGG NGG 10 8 17 8 24 0 100 5 10
    240604214951_dgeu GCCCGCCAAATACACGAGCTCTTCAGTTGTGG(+ACTCAC)GTACCAATTGGATGAGTAAGGAGAAACATGA NGG 10 8 17 8 24 0 100 5 10
    240604214949_kl5a CTCTTTTGTCTGTGGAGTCTGCTGTGTGGCAG(+ACTCAC)GATCCCAGCCCTAGAGCTGCAGGGCTGCCCT NGG 10 8 17 8 24 0 100 5 10
    240604214948_0j0a CCCACAGGGCAGCCCTGCAGCTCTAGGGCTGG(+ACTCAC)GATCCTGCCACACAGCAGACTCCACAGACAA NGG 10 8 17 8 24 0 100 5 10
    240604214946_xm8c CTGGGTGGTGGTCTGTCTGGTTCCAGTGGGAG(+ACTCAC)GTACCATCTCTTCTCCTTTGAGAAATGCCCC NGG 10 8 17 8 24 0 100 5 10
    240604214945_5ocv CTGCAGGGGCATTTCTCAAAGGAGAAGAGATG(+ACTCAC)GTACCTCCCACTGGAACCAGACAGACCACCA NGG 10 8 17 8 24 0 100 5 10
    240604214943_38b5 TCTGGGGTAGCTTACCCAGTGCTAGGCACTGG(+ACTCAC)GATCCTGAAAGTACCTTTCTTGGTGATCTGG NGG 10 8 17 8 24 0 100 5 10
    240604214942_00ny CTGTTCCAGATCACCAAGAAAGGTACTTTCAG(+ACTCAC)GATCCCAGTGCCTAGCACTGGGTAAGCTACC NGG 10 8 17 8 24 0 100 5 10
    240604214940_dfru GAAGGCAGGTCCCCAGTTGTTGGGCCAGCAGG(+ACTCAC)GTACCTTCCTTCTTCTGCAGACTGCGATAGT NGG 10 8 17 8 24 0 100 5 10
    240604214939_jyfl GTCACACTATCGCAGTCTGCAGAAGAAGGAAG(+ACTCAC)GTACCCTGCTGGCCCAACAACTGGGGACCTG NGG 10 8 17 8 24 0 100 5 10