Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213603_wnvp CAGGAAGCTGGGAGCTTGGCATAGCTTATGGG(+ACTCAC)GTACCGAGTGTCCTGTGCTCTCCTCTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240604213602_93wg TCATGCTCAAAGAGGAGAGCACAGGACACTCG(+ACTCAC)GTACCCCATAAGCTATGCCAAGCTCCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240604213600_qe9m TGTCACCAGCTGCCCTTCTCACCAGGAAACAG(+ACTCAC)GTACCTTTCCCTCTGCCTAGCGCCAACCCCA NGG 10 8 17 8 24 0 100 5 10
    240604213558_5nok CATTGTGGGGTTGGCGCTAGGCAGAGGGAAAG(+ACTCAC)GTACCTGTTTCCTGGTGAGAAGGGCAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604213556_nl3h CAACCGGCCGGAGCAGCTGGGGCAGAGGATGG(+ACTCAC)GTACCACAGGTTGAGGGGATGAAGGTCTGAT NGG 10 8 17 8 24 0 100 5 10
    240604213554_yfva CCCTCATCAGACCTTCATCCCCTCAACCTGTG(+ACTCAC)GTACCCATCCTCTGCCCCAGCTGCTCCGGCC NGG 10 8 17 8 24 0 100 5 10
    240604213553_yhs7 AACCAGGCAGCGGCTCTCTCGATGACATTCAG(+ACTCAC)GATCCCTAAAGTCATATGCTTCCCACTATAG NGG 10 8 17 8 24 0 100 5 10
    240604213551_m1js GGAAGCTATAGTGGGAAGCATATGACTTTAGG(+ACTCAC)GATCCTGAATGTCATCGAGAGAGCCGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240604213550_s3b7 GGTAGATCTGGGAAAAGGTAGGGGAGGAATAG(+ACTCAC)GATCCAGATCAAAATACACTGTGAGTATGAA NGG 10 8 17 8 24 0 100 5 10
    240604213549_zxlc TATCATTCATACTCACAGTGTATTTTGATCTG(+ACTCAC)GATCCTATTCCTCCCCTACCTTTTCCCAGAT NGG 10 8 17 8 24 0 100 5 10