Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213516_rm6r TTGCATCACTAGGCGTCTTCTGTGGATGCTGG(+ACTCAC)GATCCAAGAGAGGTCCTCGGGCTTTCATGGA NGG 10 8 17 8 24 0 100 5 10
    240604213515_raxy TGTTTTCCATGAAAGCCCGAGGACCTCTCTTG(+ACTCAC)GATCCCAGCATCCACAGAAGACGCCTAGTGA NGG 10 8 17 8 24 0 100 5 10
    240604213513_49ap ATCAGCATTGGGGTACTGACAGAAACCATAAG(+ACTCAC)GATCCTGCCCTCCACTGTTCCTTGGTTCCTA NGG 10 8 17 8 24 0 100 5 10
    240604213512_rvdl GGACATAGGAACCAAGGAACAGTGGAGGGCAG(+ACTCAC)GATCCTTATGGTTTCTGTCAGTACCCCAATG NGG 10 8 17 8 24 0 100 5 10
    240604213510_xu0v TGAGATTAATCTTTTAAAGTAGATTAACAATG(+ACTCAC)GATCCTTTGGAGCTGGAGTTACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240604213508_oggn GCTCACAACCATCTGTAACTCCAGCTCCAAAG(+ACTCAC)GATCCATTGTTAATCTACTTTAAAAGATTAA NGG 10 8 17 8 24 0 100 5 10
    240604213506_0e9t GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+ACTCAC)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604213504_v6y0 GGATGGACCATCTAGTGATTGCCATATGCAGG(+ACTCAC)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240604213501_11qt TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+ACTCAC)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240604213459_d8u0 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ACTCAC)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10