Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213426_ue0q TTGTCTATAACACAGTCCACTTAAGTAAAAGG(+ACTCAC)GTACCAAATCCCCATTGCTTTGTCCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240604213424_shvh AAAAAAAAGAAGGACAAAGCAATGGGGATTTG(+ACTCAC)GTACCCTTTTACTTAAGTGGACTGTGTTATA NGG 10 8 17 8 24 0 100 5 10
    240604213423_rjk0 ACTACATCCATGTTAAAATCCGTGTAGTCAGG(+ACTCAC)GATCCTGTAAGTCACTCCCTATGAAGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240604213421_59av CCCAATCCTGCTTCATAGGGAGTGACTTACAG(+ACTCAC)GATCCCTGACTACACGGATTTTAACATGGAT NGG 10 8 17 8 24 0 100 5 10
    240604213420_8npe CTTGCAGGTGCCCTTGGAGGCCAGGAACGTTG(+ACTCAC)GATCCCCTGGGACCTGATATTACAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240604213418_pv5y CTGACAGCCACCTGTAATATCAGGTCCCAGGG(+ACTCAC)GATCCAACGTTCCTGGCCTCCAAGGGCACCT NGG 10 8 17 8 24 0 100 5 10
    240604213417_ffwk CCCCAGGTGCCTAAATTCCAGGAGAATCACAG(+ACTCAC)GTACCAGTCTCAATTCACCACTTAACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240604213415_vhz1 ACTTCCTAAAGTTAAGTGGTGAATTGAGACTG(+ACTCAC)GTACCTGTGATTCTCCTGGAATTTAGGCACC NGG 10 8 17 8 24 0 100 5 10
    240604213414_w14e CCATCCTGGGAAAGTGACTGGGGCCCACGTGG(+ACTCAC)GATCCTAGAGCCTAGCACAGTGATATGTCCA NGG 10 8 17 8 24 0 100 5 10
    240604213412_xyyc TGCTTTGGACATATCACTGTGCTAGGCTCTAG(+ACTCAC)GATCCCACGTGGGCCCCAGTCACTTTCCCAG NGG 10 8 17 8 24 0 100 5 10