Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604214212_adhy ACTCTAGAGAACCCTGACTAATACAGAAGTTG(+ACTCAC)GTACCAGGAGTGGTTCTAGAGTAACAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240604214211_mawm TTCTTTAAAGTTCTGGAGAATGGGTGAAGCTG(+ACTCAC)GTACCTTGCAGAGAAGGGACACAAGGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240604214209_bg7d GTGGCATCTTCCTTGTGTCCCTTCTCTGCAAG(+ACTCAC)GTACCAGCTTCACCCATTCTCCAGAACTTTA NGG 10 8 17 8 24 0 100 5 10
    240604214207_supw AAAAACTTCAGAGTTGCAGACAGGACAGTCAG(+ACTCAC)GTACCCCATGGGAGTTCCTAGGGCCTGCGTA NGG 10 8 17 8 24 0 100 5 10
    240604214205_25zf ATCCATACGCAGGCCCTAGGAACTCCCATGGG(+ACTCAC)GTACCTGACTGTCCTGTCTGCAACTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604214203_s7lj ATGCGCTCTGCACTGCAGCCCCATCTATTCAG(+ACTCAC)GATCCTGCCACCCATGAGGAGATGCCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604214201_u6k7 TCCCGTGCTGGGCATCTCCTCATGGGTGGCAG(+ACTCAC)GATCCTGAATAGATGGGGCTGCAGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604214200_rrwn GCTGCAGCCCCTGCAGCCCCAACCGCAAGCTG(+ACTCAC)GATCCGGGATCTGCACATCAGCAGCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240604214158_tq4p GGGCTCCAAAGGCTGCTGATGTGCAGATCCCG(+ACTCAC)GATCCAGCTTGCGGTTGGGGCTGCAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240604214156_4nkr ACACTAAATAAAACCAAGCCAATAGTCTTGTG(+ACTCAC)GATCCCTGAATATTTCTCATTCCACACTTCA NGG 10 8 17 8 24 0 100 5 10