Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213311_2bqe AAACATTCCTAAATCATGGACCCACTGGGCAG(+ACTCAC)GATCCCTATGGGGGAGGATTCCATACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240604213309_wsw6 TAAAACTCAAGTATGGAATCCTCCCCCATAGG(+ACTCAC)GATCCTGCCCAGTGGGTCCATGATTTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604213307_xjro GATAAAAACTAAGAACAGAAACCCCACGAAAG(+ACTCAC)GATCCTACTTCTCGTCTCTCATTGCAGGATA NGG 10 8 17 8 24 0 100 5 10
    240604213306_68l3 AGATATATCCTGCAATGAGAGACGAGAAGTAG(+ACTCAC)GATCCTTTCGTGGGGTTTCTGTTCTTAGTTT NGG 10 8 17 8 24 0 100 5 10
    240604213304_8cp8 TATTGAATGCATTGTATTAAAGAAGCACCATG(+ACTCAC)GTACCTCCTGTTTGAAGTTAATTGTTCATGC NGG 10 8 17 8 24 0 100 5 10
    240604213303_5zqh AATGTGCATGAACAATTAACTTCAAACAGGAG(+ACTCAC)GTACCATGGTGCTTCTTTAATACAATGCATT NGG 10 8 17 8 24 0 100 5 10
    240604213301_lsud TATTGAATGCATTGTATTAAAGAAGCACCATG(+ACTCAC)GTACCTCCTGTTTGAAGTTAATTGTTCATGC NGG 10 8 17 8 24 0 100 5 10
    240604213259_l5sz AATGTGCATGAACAATTAACTTCAAACAGGAG(+ACTCAC)GTACCATGGTGCTTCTTTAATACAATGCATT NGG 10 8 17 8 24 0 100 5 10
    240604213258_ho55 CTTAATGCCCAGTGTGTTTGGCAATATTTCAG(+ACTCAC)GATCCGAAAATTATTCAAATTTTGTTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240604213257_xyoo TTTAATAACAAACAAAATTTGAATAATTTTCG(+ACTCAC)GATCCTGAAATATTGCCAAACACACTGGGCA NGG 10 8 17 8 24 0 100 5 10