Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213225_c039 GCTGTCTTCAGACACACCAGAAGAGGGCATTG(+ACTCAC)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604213223_c909 ACCACATGGTGGCTCACAACCATCTGTAATGG(+ACTCAC)GATCCAATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604213221_rwsb TTGATCCTGACTGTTCCTCAGAGAGGGCAACG(+ACTCAC)GATCCCTGCTCAGTTTGCTGGCTCTTGTCAC NGG 10 8 17 8 24 0 100 5 10
    240604213220_vzjz GAGAAGTGACAAGAGCCAGCAAACTGAGCAGG(+ACTCAC)GATCCGTTGCCCTCTCTGAGGAACAGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240604213218_80at GGAGGTTCAGTGACCAGTAGGCCTCCCCACTG(+ACTCAC)GTACCACCACTTCCACTGTGAGACTGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240604213216_k9rv TTGAGTTCTGCAGTCTCACAGTGGAAGTGGTG(+ACTCAC)GTACCAGTGGGGAGGCCTACTGGTCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240604213214_4trs AAGATTCTAAAGAGCGTTGAAGTGTTTAAAAG(+ACTCAC)GATCCACGAGGACAAGTTAGGTTGATGGTAT NGG 10 8 17 8 24 0 100 5 10
    240604213213_rn9q GATACATACCATCAACCTAACTTGTCCTCGTG(+ACTCAC)GATCCTTTTAAACACTTCAACGCTCTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240604213209_45db GTGAGTGACTTGGGGGTGGGTTTGAGTCTGGG(+ACTCAC)GTACCCAGTGACACAATTTTCTCATCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240604213207_yxv7 TAGAGTTCCAGATGAGAAAATTGTGTCACTGG(+ACTCAC)GTACCCCAGACTCAAACCCACCCCCAAGTCA NGG 10 8 17 8 24 0 100 5 10