Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213204_h55t GAAACGTCCTTCCTCCCCTTCGCCCTGCCTGG(+ACTCAC)GATCCAGGTAGGGTGGTTGGACCTCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240604213202_tea4 ACAAGGATGGAGAGGTCCAACCACCCTACCTG(+ACTCAC)GATCCCAGGCAGGGCGAAGGGGAGGAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240604213201_dftb GAATGCCCTGGCATCTTGGTGGCATCTTGATG(+ACTCAC)GATCCAGCTCAGTCAGGACAGCTTTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240604213200_bc75 GCTCTGTGCTCAAAGCTGTCCTGACTGAGCTG(+ACTCAC)GATCCATCAAGATGCCACCAAGATGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240604213158_8z0n CTCCTTGCAGGAGGGGATACCACCATTTCAGG(+ACTCAC)GATCCACAATTGAACCTCTGGCTTGATCCCT NGG 10 8 17 8 24 0 100 5 10
    240604213157_vkus CAGGGAGGGATCAAGCCAGAGGTTCAATTGTG(+ACTCAC)GATCCCTGAAATGGTGGTATCCCCTCCTGCA NGG 10 8 17 8 24 0 100 5 10
    240604213154_0vok GCCATCTACTGAGCGAGTGTGAACTGGCCAGG(+ACTCAC)GATCCACCAGGAAGAGGCATCATCTTCATTA NGG 10 8 17 8 24 0 100 5 10
    240604213153_ambb CAATATAATGAAGATGATGCCTCTTCCTGGTG(+ACTCAC)GATCCCTGGCCAGTTCACACTCGCTCAGTAG NGG 10 8 17 8 24 0 100 5 10
    240604213151_2z6v ACCATGGAATGGATGCCCTGGTGTATTGTCTG(+ACTCAC)GATCCCCAGAAATACTTATCACCCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240604213149_w4fv GTCCAGTGGCTGGGGTGATAAGTATTTCTGGG(+ACTCAC)GATCCAGACAATACACCAGGGCATCCATTCC NGG 10 8 17 8 24 0 100 5 10