Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213633_4z7q GAGACGCTCTTAGGGGGAGCACGACTTTCATG(+ACTCAC)GATCCTGGGGCCTGGGTGGGCTGGGGGACAG NGG 10 8 17 8 24 0 100 5 10
    240604213630_q4zi CTCAGCTGTCCCCCAGCCCACCCAGGCCCCAG(+ACTCAC)GATCCATGAAAGTCGTGCTCCCCCTAAGAGC NGG 10 8 17 8 24 0 100 5 10
    240604213628_u18w CATAATATAAGCACTTATATCCATAATTGTGG(+ACTCAC)GTACCAGTGCAAAACCATCTTGGTACATTTC NGG 10 8 17 8 24 0 100 5 10
    240604213627_b5j6 ATCAGGAAATGTACCAAGATGGTTTTGCACTG(+ACTCAC)GTACCCACAATTATGGATATAAGTGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240604213625_v294 TGAAATTAAAGGCAGACCGGTTGGGTGCTAAG(+ACTCAC)GATCCCTGGTGTGGAGAGGCCATTTCATTGA NGG 10 8 17 8 24 0 100 5 10
    240604213623_zuqw GCTGCTCAATGAAATGGCCTCTCCACACCAGG(+ACTCAC)GATCCTTAGCACCCAACCGGTCTGCCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240604213622_ca57 AATCTCTGATGTGGATTTGGTGATTTACTAAG(+ACTCAC)GTACCGTGGGAAACAAGGAAAGTGTACTTGT NGG 10 8 17 8 24 0 100 5 10
    240604213618_gt8t GTGGTACAAGTACACTTTCCTTGTTTCCCACG(+ACTCAC)GTACCTTAGTAAATCACCAAATCCACATCAG NGG 10 8 17 8 24 0 100 5 10
    240604213617_0nun ACAAACCTGAGAGCCCTGCCCAGAGTTCAGTG(+ACTCAC)GATCCTAATGAACTCCAAGAGTAATTCATTC NGG 10 8 17 8 24 0 100 5 10
    240604213616_rmph TGAGGGAATGAATTACTCTTGGAGTTCATTAG(+ACTCAC)GATCCACTGAACTCTGGGCAGGGCTCTCAGG NGG 10 8 17 8 24 0 100 5 10