Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213148_4226 CAGAGGCACATCCTTTTCCGTGGCAGCATCAG(+ACTCAC)GATCCACTCATGTTGCTGCTGGATGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604213146_ybu9 GCTCTGCCCTCCATCCAGCAGCAACATGAGTG(+ACTCAC)GATCCTGATGCTGCCACGGAAAAGGATGTGC NGG 10 8 17 8 24 0 100 5 10
    240604213145_b0ov AACCTTGTGAGCAGCCAATCCAGTTCTAGCTG(+ACTCAC)GATCCCTCCACCCCACCCCCACCCCACCCCA NGG 10 8 17 8 24 0 100 5 10
    240604213143_vior TGGGGTGGGGTGGGGTGGGGGTGGGGTGGAGG(+ACTCAC)GATCCAGCTAGAACTGGATTGGCTGCTCACA NGG 10 8 17 8 24 0 100 5 10
    240604213141_w97e TCTGTGTGAGTGTACACCACTTGTGTGGAGAG(+ACTCAC)GTACCCATGGAGCCCAGAGGTAGTGTATCCC NGG 10 8 17 8 24 0 100 5 10
    240604213139_yzvj TCCAGGGGATACACTACCTCTGGGCTCCATGG(+ACTCAC)GTACCTCTCCACACAAGTGGTGTACACTCAC NGG 10 8 17 8 24 0 100 5 10
    240604213138_c12z TGCACACACCCATCTTCGCTACTCCAGACTAG(+ACTCAC)GATCCATAGCAATTGGGAAATGGGCATTGGG NGG 10 8 17 8 24 0 100 5 10
    240604213137_n1ma TACTCCCCAATGCCCATTTCCCAATTGCTATG(+ACTCAC)GATCCTAGTCTGGAGTAGCGAAGATGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240604213135_xb4i CCCAGGAAATGCCTCCCTCCGGTCCAGGCAGG(+ACTCAC)GATCCCAGCACACACACCCCTGCTGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240604213133_6hqv CCCTCCACCACCAGCAGGGGTGTGTGTGCTGG(+ACTCAC)GATCCCTGCCTGGACCGGAGGGAGGCATTTC NGG 10 8 17 8 24 0 100 5 10