Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213240_pwn8 GAATCAGATAACAGAATCAGTGTTTCAGTCAG(+ACTCAC)GATCCACACTGCCTGCCAGGCTGTGCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240604213239_u661 CACATCCACAGCACAGCCTGGCAGGCAGTGTG(+ACTCAC)GATCCTGACTGAAACACTGATTCTGTTATCT NGG 10 8 17 8 24 0 100 5 10
    240604213237_uixk CGGTGAAGCCTTACGACTCCACCACAGTTCGG(+ACTCAC)GATCCTAGATTCTGGCGTACATAGCTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240604213236_qgh4 AGAGTTGCAGAGCTATGTACGCCAGAATCTAG(+ACTCAC)GATCCCGAACTGTGGTGGAGTCGTAAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240604213234_3rbr TGGTGCCATCCTAGGGACCAAGGTCTTTACAG(+ACTCAC)GTACCTGGGCCTGTGGGAGACATTGTTTACA NGG 10 8 17 8 24 0 100 5 10
    240604213232_9wvb TTGAGTGTAAACAATGTCTCCCACAGGCCCAG(+ACTCAC)GTACCTGTAAAGACCTTGGTCCCTAGGATGG NGG 10 8 17 8 24 0 100 5 10
    240604213231_sv2g CGGCGTCCTTCACTGATCGTTCTGTTTTTGAG(+ACTCAC)GATCCCTGTGGCCCCGGGATCAGCCCATCAC NGG 10 8 17 8 24 0 100 5 10
    240604213229_zpdi AAGGAGTGATGGGCTGATCCCGGGGCCACAGG(+ACTCAC)GATCCTCAAAAACAGAACGATCAGTGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604213227_g58m CTAAGTAGCAGCCCTGACTTCTGGTCAGGTAG(+ACTCAC)GATCCAACTGGCAGGAACCAGGCCAAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240604213226_ganj ATGTGACTCCTTGGCCTGGTTCCTGCCAGTTG(+ACTCAC)GATCCTACCTGACCAGAAGTCAGGGCTGCTA NGG 10 8 17 8 24 0 100 5 10