Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213342_thna AATAATGTTAACATTATTATATAAACAACTTG(+ACTCAC)GATCCCAAATTCTTGGAAGTTCATGTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240604213340_q4af ACGTTTTTACACATGAACTTCCAAGAATTTGG(+ACTCAC)GATCCAAGTTGTTTATATAATAATGTTAACA NGG 10 8 17 8 24 0 100 5 10
    240604213339_pj5u TTTAGACCTCCATAGCTACAAATTTAAAATAG(+ACTCAC)GTACCTTCCACATTGTCCAATGAATCCTATG NGG 10 8 17 8 24 0 100 5 10
    240604213337_aggz GACTCCATAGGATTCATTGGACAATGTGGAAG(+ACTCAC)GTACCTATTTTAAATTTGTAGCTATGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240604213335_9nb2 AAAAACAAAATTAAACTCTGTCGAATCGCTGG(+ACTCAC)GATCCACTCGCTGTTCAAGGCTCCTCTAAGT NGG 10 8 17 8 24 0 100 5 10
    240604213334_l8yj CAAGCACTTAGAGGAGCCTTGAACAGCGAGTG(+ACTCAC)GATCCCAGCGATTCGACAGAGTTTAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240604213333_07ff AATAAAGATTGCAGAAGCCAATTGGTTTTCTG(+ACTCAC)GATCCCTAGAGGAAGAGGAGACAACGATATA NGG 10 8 17 8 24 0 100 5 10
    240604213331_v86g TTCTTTATATCGTTGTCTCCTCTTCCTCTAGG(+ACTCAC)GATCCAGAAAACCAATTGGCTTCTGCAATCT NGG 10 8 17 8 24 0 100 5 10
    240604213330_azyr ATTATTGAACTATTCATTCATTTAGCCTAAAG(+ACTCAC)GATCCAAGGTGTTAGAGTTTCTTTCCCTATG NGG 10 8 17 8 24 0 100 5 10
    240604213328_kljh TGGGGCATAGGGAAAGAAACTCTAACACCTTG(+ACTCAC)GATCCTTTAGGCTAAATGAATGAATAGTTCA NGG 10 8 17 8 24 0 100 5 10