Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213410_5tk7 TGTACTTCCTTCATCCGGTGGGTTCCCTGCTG(+ACTCAC)GTACCACTCTCCAGAATCCATACATGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604213409_w7h1 ATCAACACACCATGTATGGATTCTGGAGAGTG(+ACTCAC)GTACCAGCAGGGAACCCACCGGATGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604213408_b4tj GTTTCTAGATGAAAGGGGATTTCTATTCAGAG(+ACTCAC)GTACCTGTGTGTATCTGTTAGTAACCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604213407_7uvt TAGTCCCTGGGGTTACTAACAGATACACACAG(+ACTCAC)GTACCTCTGAATAGAAATCCCCTTTCATCTA NGG 10 8 17 8 24 0 100 5 10
    240604213405_x1gs GGGAAGTCATGGCATGTTACTGCAAAGGTCTG(+ACTCAC)GTACCCTCAACTCATAGAGTTGACAGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240604213404_cc0b TGTCCCTAACCTGTCAACTCTATGAGTTGAGG(+ACTCAC)GTACCAGACCTTTGCAGTAACATGCCATGAC NGG 10 8 17 8 24 0 100 5 10
    240604213402_8b7x GGTCAGGTTGTCACAGGAATACCCCATTCCTG(+ACTCAC)GTACCAATCTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240604213401_t6nn GCAGGAATAGAAACCCTGACTAAGACAGATTG(+ACTCAC)GTACCAGGAATGGGGTATTCCTGTGACAACC NGG 10 8 17 8 24 0 100 5 10
    240604213359_fy8y ATGGAACCAGCGTCTGTGAAAAGTCAGGATTG(+ACTCAC)GTACCAACACGTGTGAGTGAAAACTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604213358_kiq4 AGAAATCCCAGAGTTTTCACTCACACGTGTTG(+ACTCAC)GTACCAATCCTGACTTTTCACAGACGCTGGT NGG 10 8 17 8 24 0 100 5 10