Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213056_2wzo TTGATTCTTTCAGGATGTGACGCCCACTCTGG(+ACTCAC)GATCCAGTCCTGGTTGATGTCAGCATGCCAC NGG 10 8 17 8 24 0 100 5 10
    240604213054_i8ul TGGAGGTGGCATGCTGACATCAACCAGGACTG(+ACTCAC)GATCCCAGAGTGGGCGTCACATCCTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604213053_ylwv CCTGAGTATACCTGAGTCAGAGCGTGGAATCG(+ACTCAC)GATCCAAGAAGTCAGTCGGCCTGGGGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240604213052_hu70 ATCGACTGGACCCCAGGCCGACTGACTTCTTG(+ACTCAC)GATCCGATTCCACGCTCTGACTCAGGTATAC NGG 10 8 17 8 24 0 100 5 10
    240604213050_unfi CTATGACATCTGTGTGTCTGTGGTCTCACGAG(+ACTCAC)GTACCTGGAGGTGTATGTGCACGGACATGGG NGG 10 8 17 8 24 0 100 5 10
    240604213048_9k7e TACTTCCCATGTCCGTGCACATACACCTCCAG(+ACTCAC)GTACCTCGTGAGACCACAGACACACAGATGT NGG 10 8 17 8 24 0 100 5 10
    240604213047_mdap GAAAGGAAGGAAAGGGGTTTTGAGAAGAAAAG(+ACTCAC)GATCCAAGCAATGGGGGCAGTTCTCACACCT NGG 10 8 17 8 24 0 100 5 10
    240604213046_g3vz CTCTGAGGTGTGAGAACTGCCCCCATTGCTTG(+ACTCAC)GATCCTTTTCTTCTCAAAACCCCTTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240604213044_i8er CTGACAGTGCCTTTCTGTCTTCAAAGACACTG(+ACTCAC)GTACCAGCCAGGCAGTCAGGTGAACTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240604213043_x3q6 GCTGGGAAGGAGTTCACCTGACTGCCTGGCTG(+ACTCAC)GTACCAGTGTCTTTGAAGACAGAAAGGCACT NGG 10 8 17 8 24 0 100 5 10