Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213007_hgbt GCCATTTCTGTACACACTGTTCTCTTGTTCAG(+ACTCAC)GTACCTGGAGAGGAAGGCTAGCCTTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240604213005_3yk3 GACTGGAGAAGAAGGCTAGCCTTCCTCTCCAG(+ACTCAC)GTACCTGAACAAGAGAACAGTGTGTACAGAA NGG 10 8 17 8 24 0 100 5 10
    240604213003_zuoc GTCTGCGAGTGTGGCCGCAAGGAAAATGGGAG(+ACTCAC)GATCCAACAGTCCTCTTTATTCTTTTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240604213002_x6of GCCAGGAAGCAAAAGAATAAAGAGGACTGTTG(+ACTCAC)GATCCTCCCATTTTCCTTGCGGCCACACTCG NGG 10 8 17 8 24 0 100 5 10
    240604213001_erbj GGTTAGGAAATGATATGGTCAAAGCCAAAGTG(+ACTCAC)GATCCAACTTATTTTAAGTCCTTTTAAATGA NGG 10 8 17 8 24 0 100 5 10
    240604212959_qz1h TACCTTCATTTAAAAGGACTTAAAATAAGTTG(+ACTCAC)GATCCACTTTGGCTTTGACCATATCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240604212958_cria ACTAGGCTGCCTGGAGTCCACAGGCAGTTCTG(+ACTCAC)GATCCACCATGTAAACGACAGAAGGAACCTT NGG 10 8 17 8 24 0 100 5 10
    240604212956_renr TTGTCAAGGTTCCTTCTGTCGTTTACATGGTG(+ACTCAC)GATCCAGAACTGCCTGTGGACTCCAGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240604212955_1s2a TAAGGCACATGGAAGACTGTTTATTTGCACAG(+ACTCAC)GTACCGAAATTTTTTTAAGAACAGCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240604212954_s5qm GAGATTTGACAGCTGTTCTTAAAAAAATTTCG(+ACTCAC)GTACCTGTGCAAATAAACAGTCTTCCATGTG NGG 10 8 17 8 24 0 100 5 10