Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213452_3lqt AGGCCACTGTAAATTAAGGACTTTCTGAGAAG(+ACTCAC)GTACCCACACCTCCTTCCATGCTGGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240604213451_eowt ATTAACCTGACCCAGCATGGAAGGAGGTGTGG(+ACTCAC)GTACCTTCTCAGAAAGTCCTTAATTTACAGT NGG 10 8 17 8 24 0 100 5 10
    240604213449_6cx9 CCTCTGCTTCGGGTACGTACCCATTTCTGTGG(+ACTCAC)GTACCTATTAATATCTACTCTGGTATATGCT NGG 10 8 17 8 24 0 100 5 10
    240604213447_ivn4 AACACAGCATATACCAGAGTAGATATTAATAG(+ACTCAC)GTACCCACAGAAATGGGTACGTACCCGAAGC NGG 10 8 17 8 24 0 100 5 10
    240604213446_b7j0 AATTCTATTTTATGAGGGAGTTACTTTGTAAG(+ACTCAC)GATCCTGTCATTTTTCTGTACCCAAGGATTG NGG 10 8 17 8 24 0 100 5 10
    240604213445_0xz2 AGGTTCAATCCTTGGGTACAGAAAAATGACAG(+ACTCAC)GATCCTTACAAAGTAACTCCCTCATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240604213443_7cop AGACCTGCATATGGGGAGGTAACATTGTTGAG(+ACTCAC)GATCCCACTCTGGCATTCATGTTGTCGTAAA NGG 10 8 17 8 24 0 100 5 10
    240604213442_xib6 CTTACTTTACGACAACATGAATGCCAGAGTGG(+ACTCAC)GATCCTCAACAATGTTACCTCCCCATATGCA NGG 10 8 17 8 24 0 100 5 10
    240604213439_bzan CACTTGAGTAGAGATGCCCTGGGAGGCCAGGG(+ACTCAC)GATCCCAAGGCCCCATCAGAATCTTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604213437_z8wi AAACAAAAAACAAGATTCTGATGGGGCCTTGG(+ACTCAC)GATCCCCTGGCCTCCCAGGGCATCTCTACTC NGG 10 8 17 8 24 0 100 5 10