Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212935_gdjf CACTGCCTCTCAGTAGTTTTAAAATATCCCAG(+ACTCAC)GTACCACGTGCTTACTTTTATTACTTGGGTA NGG 10 8 17 8 24 0 100 5 10
    240604212933_7go5 TATATTACCCAAGTAATAAAAGTAAGCACGTG(+ACTCAC)GTACCTGGGATATTTTAAAACTACTGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240604212932_gc8l ACACGTAGTGATGATAGAAATTAGAATTTAAG(+ACTCAC)GATCCTATTGCTATGACTCTCATTTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604212931_6tuj AGGGCAGAGAGAAATGAGAGTCATAGCAATAG(+ACTCAC)GATCCTTAAATTCTAATTTCTATCATCACTA NGG 10 8 17 8 24 0 100 5 10
    240604212929_ciz5 CATTGCTGTGTTCGAATAAAGCAAGCCACAGG(+ACTCAC)GTACCGTGAGGGAAAGCGTGAGGCACGCCGC NGG 10 8 17 8 24 0 100 5 10
    240604212928_0n6n GTTCTGCGGCGTGCCTCACGCTTTCCCTCACG(+ACTCAC)GTACCCTGTGGCTTGCTTTATTCGAACACAG NGG 10 8 17 8 24 0 100 5 10
    240604212925_yrj9 CACCAAGCCTGATAGCCTGCATTCGATCCCGG(+ACTCAC)GATCCATGTGGTACAAGAAGAGAGTTAGAAT NGG 10 8 17 8 24 0 100 5 10
    240604212924_aynq AGTCAATTCTAACTCTCTTCTTGTACCACATG(+ACTCAC)GATCCCGGGATCGAATGCAGGCTATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240604212922_5q74 GTTGACACGTGCTGCTGTGTTTATTTAATATG(+ACTCAC)GATCCACATCAGGAAAGAGGGAATAATACAC NGG 10 8 17 8 24 0 100 5 10
    240604212921_f0ch AAAAAGTGTATTATTCCCTCTTTCCTGATGTG(+ACTCAC)GATCCATATTAAATAAACACAGCAGCACGTG NGG 10 8 17 8 24 0 100 5 10