Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212919_oxy7 TGGCAATGCCTGTTCATCACCCCTCTCTAAGG(+ACTCAC)GTACCGTGGTCAAATAAGTTGCGCTACAACT NGG 10 8 17 8 24 0 100 5 10
    240604212917_flmv TGTGAAGTTGTAGCGCAACTTATTTGACCACG(+ACTCAC)GTACCCTTAGAGAGGGGTGATGAACAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604212916_phtk GGTTTTCTAATGAAAACAAAAATGGATTTCAG(+ACTCAC)GTACCATGCTGATATTTGTTTTGTCTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240604212914_pm6d CGTCTAAAACAGACAAAACAAATATCAGCATG(+ACTCAC)GTACCTGAAATCCATTTTTGTTTTCATTAGA NGG 10 8 17 8 24 0 100 5 10
    240604212913_eu8j GTCCGTTGGTAAAACCTGTTTACAGTTGGCAG(+ACTCAC)GATCCTCTCATGAAGCTTACTATTGAGGCAA NGG 10 8 17 8 24 0 100 5 10
    240604212912_m7dv TGACTTTGCCTCAATAGTAAGCTTCATGAGAG(+ACTCAC)GATCCTGCCAACTGTAAACAGGTTTTACCAA NGG 10 8 17 8 24 0 100 5 10
    240604212910_9a2t TCCCACAAACATCTGTCTTGCCAGCTGGGCAG(+ACTCAC)GATCCAATTCCCCATACTGGCTTGTATACCC NGG 10 8 17 8 24 0 100 5 10
    240604212909_05dh TTTGAGGGTATACAAGCCAGTATGGGGAATTG(+ACTCAC)GATCCTGCCCAGCTGGCAAGACAGATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240604212907_ki8v GTAAATGTATTTTTCCCTTTTAACTGTTCTTG(+ACTCAC)GATCCAAAGCATATGACCACTGCTCATTGAC NGG 10 8 17 8 24 0 100 5 10
    240604212906_6fk4 TCAGTGTCAATGAGCAGTGGTCATATGCTTTG(+ACTCAC)GATCCAAGAACAGTTAAAAGGGAAAAATACA NGG 10 8 17 8 24 0 100 5 10