Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212819_y642 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ACTCAC)GATCCCTGGATAAGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604212817_hd4q GGATGGACCATGTAGAGACTGCCTTATCCAGG(+ACTCAC)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240604212816_ssd4 TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ACTCAC)GATCCTCCGGTAGTACTATGTCCAATTTACT NGG 10 8 17 8 24 0 100 5 10
    240604212814_18q1 TCCTCAGTAAATTGGACATAGTACTACCGGAG(+ACTCAC)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240604212812_w9a1 TTCTGGATATATGCCCAGGAGAGGTATTGTGG(+ACTCAC)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240604212809_ojep TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ACTCAC)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240604212808_ty71 GTCAGCTCTGGGGTGCAGATACAAACCAGAGG(+ACTCAC)GATCCTGTCTTCTGCCATTCCTTGGTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240604212806_lqmk TAACACAGGAACCAAGGAATGGCAGAAGACAG(+ACTCAC)GATCCCTCTGGTTTGTATCTGCACCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604212803_z0u4 TCCTCATAAAATTGGACATAGTACTACCGGAG(+ACTCAC)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240604212802_cz8b TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ACTCAC)GATCCTCCGGTAGTACTATGTCCAATTTTAT NGG 10 8 17 8 24 0 100 5 10