Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212708_ti7w GTGGTGCACTCCAACAGCATTTTAAGTACTTG(+ACTCAC)GATCCCACGTTGATCTTATAAACACAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240604212706_scl3 TTTTATTGCTTGTGTTTATAAGATCAACGTGG(+ACTCAC)GATCCAAGTACTTAAAATGCTGTTGGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240604212704_tmql TCTCTGTCTGACTTCCTATGCATGGGGGCTGG(+ACTCAC)GATCCATTTCTAAGGAGAATGGATAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604212703_8tuq GCCTACTTCCTTATCCATTCTCCTTAGAAATG(+ACTCAC)GATCCCAGCCCCCATGCATAGGAAGTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240604212701_z5xl GTGGTGCACTCCAACAGCATTTTAAGTACTTG(+ACTCAC)GATCCCACGTTGATCTTATAAACACAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240604212700_vsz5 TTTTATTGCTTGTGTTTATAAGATCAACGTGG(+ACTCAC)GATCCAAGTACTTAAAATGCTGTTGGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240604212658_qptr TCTCTGTCTGACTTCCTATGCATGGGGGCTGG(+ACTCAC)GATCCATTTCTAAGGAGAATGGATAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604212657_0hf8 GCCTACTTCCTTATCCATTCTCCTTAGAAATG(+ACTCAC)GATCCCAGCCCCCATGCATAGGAAGTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240604212655_nj06 ACAAAGTCCTTTAATTTAAAAAAAAAAAAAAG(+ACTCAC)GTACCTGATTCTCACTTCTCTCAATCTTCTA NGG 10 8 17 8 24 0 100 5 10
    240604212654_5y71 GTAAATAGAAGATTGAGAGAAGTGAGAATCAG(+ACTCAC)GTACCTTTTTTTTTTTTTTAAATTAAAGGAC NGG 10 8 17 8 24 0 100 5 10