Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212636_aflk AGTGTCAGGCTTTGGGACCTCCCTTTGAGCTG(+ACTCAC)GATCCCATTTTGGGCCTGTTGCTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240604212634_w98n GAAAAGAAGGTCCAGCAACAGGCCCAAAATGG(+ACTCAC)GATCCAGCTCAAAGGGAGGTCCCAAAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604212632_59pe GACCAGCACGACTAGCACACATAGGGACCTGG(+ACTCAC)GATCCTCTGTTTCCTCCTGTCTCTAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240604212630_edqp CCTTCCTCTCCTAGAGACAGGAGGAAACAGAG(+ACTCAC)GATCCCAGGTCCCTATGTGTGCTAGTCGTGC NGG 10 8 17 8 24 0 100 5 10
    240604212629_wcjy TGAAGAAGTAGAGAAACAAGTCAGAGTGGTTG(+ACTCAC)GATCCCCTGATTTACTCTGTCAGTACATTTT NGG 10 8 17 8 24 0 100 5 10
    240604212627_n2ta AAAAAAAAATGTACTGACAGAGTAAATCAGGG(+ACTCAC)GATCCAACCACTCTGACTTGTTTCTCTACTT NGG 10 8 17 8 24 0 100 5 10
    240604212626_yrex GGGGAGACACTATGGCATATATTACAGAGAAG(+ACTCAC)GATCCTTTCTGGCTACTGGTCACTGCTACAT NGG 10 8 17 8 24 0 100 5 10
    240604212624_ebec TCTTAATGTAGCAGTGACCAGTAGCCAGAAAG(+ACTCAC)GATCCTTCTCTGTAATATATGCCATAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240604212623_apcv GGGATTCTACCGTGCACTGCTGCATCCAGTAG(+ACTCAC)GATCCTAGCTAAAGGCTTTCTCTACTATGAT NGG 10 8 17 8 24 0 100 5 10
    240604212622_rk4v ACAACATCATAGTAGAGAAAGCCTTTAGCTAG(+ACTCAC)GATCCTACTGGATGCAGCAGTGCACGGTAGA NGG 10 8 17 8 24 0 100 5 10