Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212549_ngl7 TCACAAGAACATGATCTGTCCCGGCAGTGAAG(+ACTCAC)GTACCAAGAGCAGCTTGGCCCATGGTGGCGA NGG 10 8 17 8 24 0 100 5 10
    240604212548_fr2n CCCATTCGCCACCATGGGCCAAGCTGCTCTTG(+ACTCAC)GTACCTTCACTGCCGGGACAGATCATGTTCT NGG 10 8 17 8 24 0 100 5 10
    240604212547_x89d CCTACAGTGACTTGATGTACCAGGGTGGGTTG(+ACTCAC)GTACCCAGAGGAGCCTCCATCTTCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240604212545_abmm CTTCACCTCTGAGAAGATGGAGGCTCCTCTGG(+ACTCAC)GTACCAACCCACCCTGGTACATCAAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240604212542_ljz0 TTGTAAAAGGCCTACCTTCAAGACCACCCATG(+ACTCAC)GATCCTCAAACCTATTAATCTGTTAAGGACG NGG 10 8 17 8 24 0 100 5 10
    240604212541_m9l3 GGCTCCGTCCTTAACAGATTAATAGGTTTGAG(+ACTCAC)GATCCATGGGTGGTCTTGAAGGTAGGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240604212539_c6o9 GTCACATTGTATCCAGGCAGGAAGCAGAGATG(+ACTCAC)GATCCAGGTGCTCGGTCCCCTCTCCTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240604212537_s8tb CTATAAGAACAGGAGAGGGGACCGAGCACCTG(+ACTCAC)GATCCATCTCTGCTTCCTGCCTGGATACAAT NGG 10 8 17 8 24 0 100 5 10
    240604212535_92pp GTAAACACACAAACAGTAAGCTTAGCTGTTGG(+ACTCAC)GATCCTGCCCCGAGGTCACCACTCCCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240604212534_3ewy ATGGAATGAAGGGAGTGGTGACCTCGGGGCAG(+ACTCAC)GATCCCAACAGCTAAGCTTACTGTTTGTGTG NGG 10 8 17 8 24 0 100 5 10