Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212501_yq87 GTGGGGTTCCTGCGCTGTGTGTGCCTACTCAG(+ACTCAC)GTACCCCAACATTGCTTTCCCTAGCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240604212459_onjk CACAGCAGAGAGCTAGGGAAAGCAATGTTGGG(+ACTCAC)GTACCTGAGTAGGCACACACAGCGCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240604212458_7scl GCTCAATGCCATCATCACTGTGATTAAGTGAG(+ACTCAC)GTACCAGGGTTCATAGCGTCGGAGACTGCTG NGG 10 8 17 8 24 0 100 5 10
    240604212455_hknb AGCGTCAGCAGTCTCCGACGCTATGAACCCTG(+ACTCAC)GTACCTCACTTAATCACAGTGATGATGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604212454_yehf ACAGGAACCAGTCCCTAAAAGTGCTCTTCTCG(+ACTCAC)GTACCTGGCGAGGCAATCATTCCAGCGCTTC NGG 10 8 17 8 24 0 100 5 10
    240604212452_pv2s AAACAGAAGCGCTGGAATGATTGCCTCGCCAG(+ACTCAC)GTACCGAGAAGAGCACTTTTAGGGACTGGTT NGG 10 8 17 8 24 0 100 5 10
    240604212451_qc61 AGAATCTAGTAGCCACACAATGGGGGGCTCAG(+ACTCAC)GTACCATTATTTACTACTTTCTTTTGACCTA NGG 10 8 17 8 24 0 100 5 10
    240604212450_n1l5 AGTCCTAGGTCAAAAGAAAGTAGTAAATAATG(+ACTCAC)GTACCTGAGCCCCCCATTGTGTGGCTACTAG NGG 10 8 17 8 24 0 100 5 10
    240604212448_wyq8 AAATACTCGATGTGCAATTACTTTGCACTTGG(+ACTCAC)GATCCAGCAAGCCCCTCAGCGGTATCCTGAT NGG 10 8 17 8 24 0 100 5 10
    240604212447_zcc2 TGGTTATCAGGATACCGCTGAGGGGCTTGCTG(+ACTCAC)GATCCCAAGTGCAAAGTAATTGCACATCGAG NGG 10 8 17 8 24 0 100 5 10