Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212445_e6sk AATGACATGAACTTATTAAGAATTAACTTAAG(+ACTCAC)GATCCAATATATATATATATATATAATATGT NGG 10 8 17 8 24 0 100 5 10
    240604212444_im9h ATATAACATATTATATATATATATATATATTG(+ACTCAC)GATCCTTAAGTTAATTCTTAATAAGTTCATG NGG 10 8 17 8 24 0 100 5 10
    240604212443_aqbm TGCTTAATTCTTTTAGCGAATGCACAGGTTAG(+ACTCAC)GTACCACCGTGAGGAAAAGGAGGGTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240604212441_q3b6 TCATTCCCCACACCCTCCTTTTCCTCACGGTG(+ACTCAC)GTACCTAACCTGTGCATTCGCTAAAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240604212440_m64u TGCCTTCATCTCAGAATTGCTCATCCCAGGTG(+ACTCAC)GATCCCATGTGTCTGCAGTAGACCAGTCACC NGG 10 8 17 8 24 0 100 5 10
    240604212438_k5s8 ACAAAGGTGACTGGTCTACTGCAGACACATGG(+ACTCAC)GATCCACCTGGGATGAGCAATTCTGAGATGA NGG 10 8 17 8 24 0 100 5 10
    240604212436_o196 CACTTTGTAAGGAAAGTGGATACTTTAGAATG(+ACTCAC)GTACCCTATGCATCCAAATGAAATGTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240604212434_656c ATGTAAAGGCACATTTCATTTGGATGCATAGG(+ACTCAC)GTACCATTCTAAAGTATCCACTTTCCTTACA NGG 10 8 17 8 24 0 100 5 10
    240604212433_fyoh AGACTAAGCAATAATATTTAGCTCTGGAATGG(+ACTCAC)GTACCATCTCCTTTTTCCTCATTAAAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240604212431_6dr6 TGGTTGAGCCTTTAATGAGGAAAAAGGAGATG(+ACTCAC)GTACCCATTCCAGAGCTAAATATTATTGCTT NGG 10 8 17 8 24 0 100 5 10