Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212359_qmce ACAAACACACAAATAATAATACAAATCCAGTG(+ACTCAC)GATCCTTCACGTCTAATGATAAACTGAGAAC NGG 10 8 17 8 24 0 100 5 10
    240604212357_hums ATGTAGTTCTCAGTTTATCATTAGACGTGAAG(+ACTCAC)GATCCACTGGATTTGTATTATTATTTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604212355_doe7 ACTGGCCCAGTCTTGTGAAGACTAGCTCCGTG(+ACTCAC)GTACCTCCCCAAGTTAGAAGACGTGCTCATT NGG 10 8 17 8 24 0 100 5 10
    240604212353_5i77 GTTCCAATGAGCACGTCTTCTAACTTGGGGAG(+ACTCAC)GTACCACGGAGCTAGTCTTCACAAGACTGGG NGG 10 8 17 8 24 0 100 5 10
    240604212351_mtl1 TTGGTGGCACAAAGCAGCAATGTGACCTAGTG(+ACTCAC)GATCCGAAAGGGACGGGATCTGAAATTACCC NGG 10 8 17 8 24 0 100 5 10
    240604212350_xck1 GAGAAGGGTAATTTCAGATCCCGTCCCTTTCG(+ACTCAC)GATCCACTAGGTCACATTGCTGCTTTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604212348_rmo0 AACTTACATATTTATTGCCCAGGTGTAGGGAG(+ACTCAC)GATCCATCACGATGTCAGTTCTTGGTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240604212346_8zcy ACAGGGGAAAACCAAGAACTGACATCGTGATG(+ACTCAC)GATCCTCCCTACACCTGGGCAATAAATATGT NGG 10 8 17 8 24 0 100 5 10
    240604212344_v7kq CAAACCCACCACGACTTTTACAGCAGAAGTGG(+ACTCAC)GTACCACTCCTTGGATCATGAGGCCCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240604212343_v16e TGCTTCCCAAGGGCCTCATGATCCAAGGAGTG(+ACTCAC)GTACCCACTTCTGCTGTAAAAGTCGTGGTGG NGG 10 8 17 8 24 0 100 5 10