Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212607_gdpz AAAGAACATGAAAAATTCAGAGGAGAAATGAG(+ACTCAC)GTACCCATAGTCAACAGAGCGTTTTACTGAG NGG 10 8 17 8 24 0 100 5 10
    240604212605_3vvx TGTATCTCAGTAAAACGCTCTGTTGACTATGG(+ACTCAC)GTACCTCATTTCTCCTCTGAATTTTTCATGT NGG 10 8 17 8 24 0 100 5 10
    240604212603_c6qd TGGTACTAGGGTTTCCTGTAAACACCTTGTGG(+ACTCAC)GTACCTCATAAGCAGCTGAGATTATTAGTGT NGG 10 8 17 8 24 0 100 5 10
    240604212602_hy0v ACCAAACACTAATAATCTCAGCTGCTTATGAG(+ACTCAC)GTACCCACAAGGTGTTTACAGGAAACCCTAG NGG 10 8 17 8 24 0 100 5 10
    240604212600_qno0 TGAAAATACTTGAAGTATTTCCTGACCTCCAG(+ACTCAC)GTACCACTTTAAGCATGCTAGATATCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240604212558_w8gb TCCCAACCTAGATATCTAGCATGCTTAAAGTG(+ACTCAC)GTACCTGGAGGTCAGGAAATACTTCAAGTAT NGG 10 8 17 8 24 0 100 5 10
    240604212557_jxyt CTCTAGTGCTTTATAGCAAAAGCTATAAAGTG(+ACTCAC)GTACCCGGGTGACCAGCATTGCCATGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240604212554_0o94 TGGATGTAACCATGGCAATGCTGGTCACCCGG(+ACTCAC)GTACCACTTTATAGCTTTTGCTATAAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240604212553_ixfz CCACGCTGCTTCAAGCTAACTGCACCAGATTG(+ACTCAC)GATCCAGCCATCAGCACTCCCAGAGACTCCT NGG 10 8 17 8 24 0 100 5 10
    240604212551_y8x6 TTTTAAGGAGTCTCTGGGAGTGCTGATGGCTG(+ACTCAC)GATCCAATCTGGTGCAGTTAGCTTGAAGCAG NGG 10 8 17 8 24 0 100 5 10