Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212850_kv5m CCAGACAAATGTAGTTTACTGCAGGCTTACTG(+ACTCAC)GATCCTGACATTTAATTAAATATAACATAAT NGG 10 8 17 8 24 0 100 5 10
    240604212849_wydu ACTAGATTATGTTATATTTAATTAAATGTCAG(+ACTCAC)GATCCAGTAAGCCTGCAGTAAACTACATTTG NGG 10 8 17 8 24 0 100 5 10
    240604212847_y5f9 ATAACATGGCAATAATGAAGTGCTGTTTCCTG(+ACTCAC)GTACCTTGGGAATACTAAAGAAAGAAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240604212844_pjve GCAATGCTTTTTCTTTCTTTAGTATTCCCAAG(+ACTCAC)GTACCAGGAAACAGCACTTCATTATTGCCAT NGG 10 8 17 8 24 0 100 5 10
    240604212843_09wn TTAAAAGAGAAACCAGAAACATCTAATCTGAG(+ACTCAC)GATCCAGACAATAAATATTACATGGATCATT NGG 10 8 17 8 24 0 100 5 10
    240604212842_4kd6 CTCGGAATGATCCATGTAATATTTATTGTCTG(+ACTCAC)GATCCTCAGATTAGATGTTTCTGGTTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604212840_6k2u TTAGATTATGTATGAAAATTCATACAATACTG(+ACTCAC)GATCCTATGACTAAAATGAAAATAGTAGGAG NGG 10 8 17 8 24 0 100 5 10
    240604212839_lfu2 TGCTGCTCCTACTATTTTCATTTTAGTCATAG(+ACTCAC)GATCCAGTATTGTATGAATTTTCATACATAA NGG 10 8 17 8 24 0 100 5 10
    240604212838_9wix GTCCCTGGTTCTGCTCAAACAGTTACTTCAAG(+ACTCAC)GTACCCTTAAAAGGGCTCCATTAAACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240604212835_q0h4 GTACATTCTTGTTTAATGGAGCCCTTTTAAGG(+ACTCAC)GTACCTTGAAGTAACTGTTTGAGCAGAACCA NGG 10 8 17 8 24 0 100 5 10