Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604213025_33j1 CATTGCATCATCTTTACATGAATGAGAAAAAG(+ACTCAC)GTACCTGTTGCACGTGGAAACCTTTAACCCA NGG 10 8 17 8 24 0 100 5 10
    240604213023_93mx GGATTTGGGTTAAAGGTTTCCACGTGCAACAG(+ACTCAC)GTACCTTTTTCTCATTCATGTAAAGATGATG NGG 10 8 17 8 24 0 100 5 10
    240604213021_9zad CAGGTGGCTCACAGACACCTATAGCTCCAGGG(+ACTCAC)GATCCTACACCCTCTTTTGAGCGCCGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240604213020_ctiq TGCATGCCCACGGCGCTCAAAAGAGGGTGTAG(+ACTCAC)GATCCCCTGGAGCTATAGGTGTCTGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604213018_si8b GCTTTGAACTTGCAGAGATCTGCCAGTCTGTG(+ACTCAC)GATCCAGGCTGCTGGGATGCAAGTACACCAC NGG 10 8 17 8 24 0 100 5 10
    240604213016_765f TTTTTGTGGTGTACTTGCATCCCAGCAGCCTG(+ACTCAC)GATCCACAGACTGGCAGATCTCTGCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240604213015_5j43 ATCTACAAGGACTTGTTCACTTCAAAGGAAGG(+ACTCAC)GATCCCATCGCTGCTATGAATCTGAAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604213013_tdvr TTCTTCACACTTCAGATTCATAGCAGCGATGG(+ACTCAC)GATCCCTTCCTTTGAAGTGAACAAGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240604213011_2vnv TGGTTTCTCCAGAAGGCGGTACATCGCTAGGG(+ACTCAC)GTACCTATAGAATACAAGGTAGGTGTCACTC NGG 10 8 17 8 24 0 100 5 10
    240604213009_7gmd TTTAAGAGTGACACCTACCTTGTATTCTATAG(+ACTCAC)GTACCCCTAGCGATGTACCGCCTTCTGGAGA NGG 10 8 17 8 24 0 100 5 10