Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211547_j9vn ATCCTGGCTTAATCTTGAAGGGCTAGTGTTAG(+ACTCAC)GATCCAGCTGAGGCAGTAAGCAAGTGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240604211545_j4ec TATTTTTTAAAAATTATTTTTGTGGACATGGG(+ACTCAC)GATCCCGTGACGGCACCTACTTTTGGGAGTC NGG 10 8 17 8 24 0 100 5 10
    240604211544_tv5z CTACAGACTCCCAAAAGTAGGTGCCGTCACGG(+ACTCAC)GATCCCCATGTCCACAAAAATAATTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240604211541_j4tb TTTTCTACAGTCCTCCCCTGTTGTACCTAGGG(+ACTCAC)GTACCAAGAAAACATAGGAATATCTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240604211540_47ks TTATTTAGTGCAGATATTCCTATGTTTTCTTG(+ACTCAC)GTACCCCTAGGTACAACAGGGGAGGACTGTA NGG 10 8 17 8 24 0 100 5 10
    240604211539_mp5b TGGTGCTGAGCTTCTTCCATCTGCATAAGATG(+ACTCAC)GATCCACTCAGTAGATACCCCAGGATAGACC NGG 10 8 17 8 24 0 100 5 10
    240604211538_cbxy CTCAGGGTCTATCCTGGGGTATCTACTGAGTG(+ACTCAC)GATCCATCTTATGCAGATGGAAGAAGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240604211536_4qoc CCACTGAACTTGGTGGCTGGTAATCTGGCCAG(+ACTCAC)GATCCAAGAGAGGATGGTGACTTTGGACATT NGG 10 8 17 8 24 0 100 5 10
    240604211534_hgrb ACAGGAATGTCCAAAGTCACCATCCTCTCTTG(+ACTCAC)GATCCTGGCCAGATTACCAGCCACCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240604211532_howk TAACAGGGCAGAGACTTGAACAGGATACCAGG(+ACTCAC)GTACCCAGCAAAACTGCCCTTTGATTGGCCT NGG 10 8 17 8 24 0 100 5 10