Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211620_wkr4 AACTCAGCTAATGGTTGTGTATTTTAAATCTG(+ACTCAC)GATCCAGCCACCTCTGGTCACACTTACCTTT NGG 10 8 17 8 24 0 100 5 10
    240604211618_l13t AAAAGAATATACCTTCTTCTCAGCACCTCATG(+ACTCAC)GTACCTTTTCCAAAACTGACCATGTAACTGG NGG 10 8 17 8 24 0 100 5 10
    240604211617_q9ln TGTGACCAGTTACATGGTCAGTTTTGGAAAAG(+ACTCAC)GTACCATGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240604211615_gr74 AAGAAAATATGAGGCCAAGAATCTGGGGACAG(+ACTCAC)GATCCTTTTAGTCTCCATCTGAGCCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604211614_4q6c GTTAACTCTGAGGCTCAGATGGAGACTAAAAG(+ACTCAC)GATCCTGTCCCCAGATTCTTGGCCTCATATT NGG 10 8 17 8 24 0 100 5 10
    240604211612_e55y GGATGGACCATGTAGAGACTGTCATATCCAGG(+ACTCAC)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240604211609_a1kf GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ACTCAC)GATCCCTGGATATGACAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604211608_z6pd CGTGGGTGGGGTCGATGGTCAGCTACATTGAG(+ACTCAC)GATCCAAGAATGGCAGCAGTGCCTGCACCCC NGG 10 8 17 8 24 0 100 5 10
    240604211606_i5fc CCAGTGGGGTGCAGGCACTGCTGCCATTCTTG(+ACTCAC)GATCCTCAATGTAGCTGACCATCGACCCCAC NGG 10 8 17 8 24 0 100 5 10
    240604211605_7imv GCATATAAGCCCCCCTGTTCTATGAGATCTGG(+ACTCAC)GTACCCTCACTGCCTTCCCAGCTCCGAGTCT NGG 10 8 17 8 24 0 100 5 10