Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211726_14fw GGACTTCCAGGTCATTGGGCCAGGCTGTTATG(+ACTCAC)GATCCTTCCGAGGAGTCTGCTAGGTGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240604211725_d85h GTTGTGAGAGAAACTCTTCCACAACATTATGG(+ACTCAC)GATCCAAAAGGAGAGGACCCAAGGGGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240604211724_h4g6 CTCTTTTAAGCCCCTTGGGTCCTCTCCTTTTG(+ACTCAC)GATCCCATAATGTTGTGGAAGAGTTTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240604211722_c8lt GGCATTCAGAGTTGGTCATTGTGGGTAAGAGG(+ACTCAC)GTACCGGCCCACCCGAAAGCCAGCATCCACG NGG 10 8 17 8 24 0 100 5 10
    240604211720_6ic4 CTGCTCGTGGATGCTGGCTTTCGGGTGGGCCG(+ACTCAC)GTACCCTCTTACCCACAATGACCAACTCTGA NGG 10 8 17 8 24 0 100 5 10
    240604211718_xx9y TCCCCTGAGTACTGAGGCTCACCACCCAGCTG(+ACTCAC)GTACCCTCGCCCTGCCTTCTGCCTCTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604211717_17m0 TAAATCCTTCAGAGGCAGAAGGCAGGGCGAGG(+ACTCAC)GTACCAGCTGGGTGGTGAGCCTCAGTACTCA NGG 10 8 17 8 24 0 100 5 10
    240604211716_ls1v TTCATACATTTAGAAGGCATATAAAGAGATAG(+ACTCAC)GATCCCTTTAGCCCAGGCTGATCTTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240604211714_xbbw ACTACAAGTTCAAGATCAGCCTGGGCTAAAGG(+ACTCAC)GATCCTATCTCTTTATATGCCTTCTAAATGT NGG 10 8 17 8 24 0 100 5 10
    240604211712_wbeq AGTGATTAAGAGCCCTGGTTGCTCTTCCAAAG(+ACTCAC)GATCCAGGCTCGATTCCCAGAACTCACATGG NGG 10 8 17 8 24 0 100 5 10