Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211742_beav CTGGCTGTCCTGGAACTCACTTTGTAGACCAG(+ACTCAC)GATCCATAACAATATTAACTGTGGATCAGGA NGG 10 8 17 8 24 0 100 5 10
    240604211741_mhnn ATGATGTTACTGCTGCATCCAGCTTTTACATG(+ACTCAC)GATCCTAATTGAGGTCCTCATATTTGCATAG NGG 10 8 17 8 24 0 100 5 10
    240604211740_wu9e GCCTCCTATGCAAATATGAGGACCTCAATTAG(+ACTCAC)GATCCATGTAAAAGCTGGATGCAGCAGTAAC NGG 10 8 17 8 24 0 100 5 10
    240604211738_ahil GTTCTCGGGGTGGTTCCTCTGTCAGCTGCCAG(+ACTCAC)GATCCTGTGCTAGAGACAGCCTGCCAATGAC NGG 10 8 17 8 24 0 100 5 10
    240604211737_3t6r TCTCTGTCATTGGCAGGCTGTCTCTAGCACAG(+ACTCAC)GATCCTGGCAGCTGACAGAGGAACCACCCCG NGG 10 8 17 8 24 0 100 5 10
    240604211735_f614 CAGATGATGGCTCTGGTGGTCATGTTAGGAGG(+ACTCAC)GATCCTGAGGTCCCTGCTCTTCACCATGCAG NGG 10 8 17 8 24 0 100 5 10
    240604211733_2np2 AGTCTCTGCATGGTGAAGAGCAGGGACCTCAG(+ACTCAC)GATCCCTCCTAACATGACCACCAGAGCCATC NGG 10 8 17 8 24 0 100 5 10
    240604211730_52jf ATCACTCCTTAAATTTACTTCCACAATCCTGG(+ACTCAC)GTACCTTTTGCCAAGGGGCTCTCTCTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604211729_blan CTGAGTCCCAAGAGAGAGCCCCTTGGCAAAAG(+ACTCAC)GTACCCAGGATTGTGGAAGTAAATTTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604211728_8tpb ACACGGCCCACACCTAGCAGACTCCTCGGAAG(+ACTCAC)GATCCATAACAGCCTGGCCCAATGACCTGGA NGG 10 8 17 8 24 0 100 5 10