Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211845_8woe CTGTCATTTTTCCCACCTAAAAATTGATCTGG(+ACTCAC)GTACCTTCCTCTGGAGAGCTGAAGTCTGAAT NGG 10 8 17 8 24 0 100 5 10
    240604211843_m0g8 CAATTCGCTCCTTCTGTTCCCCACATTCCAGG(+ACTCAC)GATCCTTCGGAGCAGGTTCTGTACGTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240604211841_gfo9 GTGGGGCTGGACGTACAGAACCTGCTCCGAAG(+ACTCAC)GATCCCTGGAATGTGGGGAACAGAAGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240604211839_2leu TTCTTCCACCCTTCTAGCCACTCTCTGGTTTG(+ACTCAC)GTACCTCCTCAAAACATCTGGGCTCCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240604211838_38zk TGTATGCAGAGGAGCCCAGATGTTTTGAGGAG(+ACTCAC)GTACCAAACCAGAGAGTGGCTAGAAGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240604211835_8uz0 GGCTGTGTCCACACCAGCAGCAGGATCCTTGG(+ACTCAC)GTACCCTTTGCTGCTTCCTTTCCCTTCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604211834_l2pf TTGTGCCTGGAAGGGAAAGGAAGCAGCAAAGG(+ACTCAC)GTACCCAAGGATCCTGCTGCTGGTGTGGACA NGG 10 8 17 8 24 0 100 5 10
    240604211832_r0at GGGAAACTATTACTTCAAATGAACAGTATTGG(+ACTCAC)GATCCTTCTGCCTTACTTAGTTACCTAGTAT NGG 10 8 17 8 24 0 100 5 10
    240604211831_7mwf TTCAGATACTAGGTAACTAAGTAAGGCAGAAG(+ACTCAC)GATCCCAATACTGTTCATTTGAAGTAATAGT NGG 10 8 17 8 24 0 100 5 10
    240604211829_armn TCCTGGCAGAGCTCCACACGGCCTTCCTGGTG(+ACTCAC)GATCCTAAAGGAGGGCGCATAGCAAGGTGCT NGG 10 8 17 8 24 0 100 5 10