Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211929_fz67 TCTCTCTATCCCTTAAAAAGAACTATCTCCAG(+ACTCAC)GTACCCAGTCCTGTTGTAGAAACCACCTGAT NGG 10 8 17 8 24 0 100 5 10
    240604211928_lpps TGGGCATCAGGTGGTTTCTACAACAGGACTGG(+ACTCAC)GTACCTGGAGATAGTTCTTTTTAAGGGATAG NGG 10 8 17 8 24 0 100 5 10
    240604211926_k14b TTGTTCTCCAGACCTGACCCAGGTAGGCAAGG(+ACTCAC)GTACCCTCCCAGTCTCACCTACCATACTGTG NGG 10 8 17 8 24 0 100 5 10
    240604211925_sfeq GATGGCACAGTATGGTAGGTGAGACTGGGAGG(+ACTCAC)GTACCCTTGCCTACCTGGGTCAGGTCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240604211923_xrg4 TGCTAAACCGTTAAGTAGCTGGAAGATATCTG(+ACTCAC)GATCCTTCTATGCCCTCTCACCCGTAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240604211922_wibt GAAGGGGGCCTACGGGTGAGAGGGCATAGAAG(+ACTCAC)GATCCAGATATCTTCCAGCTACTTAACGGTT NGG 10 8 17 8 24 0 100 5 10
    240604211920_knvy TTACAGTGACCCCCTTTGTTTGCAGTGAAGGG(+ACTCAC)GATCCTTTGCTTCTCGGTCCTCAACACGATG NGG 10 8 17 8 24 0 100 5 10
    240604211918_9v76 ACCCTCATCGTGTTGAGGACCGAGAAGCAAAG(+ACTCAC)GATCCCCTTCACTGCAAACAAAGGGGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240604211917_07l7 GCAAAAGTGTCTTCCCCCTCCCCCGACTTCAG(+ACTCAC)GATCCTGAAGGAAGGACTGGGTGCGGTGCGG NGG 10 8 17 8 24 0 100 5 10
    240604211915_9hp3 TCTTGCCGCACCGCACCCAGTCCTTCCTTCAG(+ACTCAC)GATCCTGAAGTCGGGGGAGGGGGAAGACACT NGG 10 8 17 8 24 0 100 5 10