Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604211828_zwb1 TCCGGAGCACCTTGCTATGCGCCCTCCTTTAG(+ACTCAC)GATCCACCAGGAAGGCCGTGTGGAGCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240604211827_uvk7 ATGTCACAGGGCTGTGTCCACACCAGCAGCAG(+ACTCAC)GATCCTTGGGTACCCTTTGCTGCTTCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240604211824_w7z6 GAAGGGAAAGGAAGCAGCAAAGGGTACCCAAG(+ACTCAC)GATCCTGCTGCTGGTGTGGACACAGCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604211823_9o6u TCTTCTGAGATAATAAGCACAATCCTGAAATG(+ACTCAC)GATCCAATAATTCAGCTAGATATTATAGATT NGG 10 8 17 8 24 0 100 5 10
    240604211822_fi91 ATACTAATCTATAATATCTAGCTGAATTATTG(+ACTCAC)GATCCATTTCAGGATTGTGCTTATTATCTCA NGG 10 8 17 8 24 0 100 5 10
    240604211821_uuwr ACTTCTTTTCTTCCTCTCCTCTAAGTGAACAG(+ACTCAC)GATCCCCTTTTTGGTCCATTTGTCCATGCCT NGG 10 8 17 8 24 0 100 5 10
    240604211819_li5w GTGAGAGGCATGGACAAATGGACCAAAAAGGG(+ACTCAC)GATCCTGTTCACTTAGAGGAGAGGAAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604211817_kmql TTGAGAATATAACAAAACAGCACTTTCTTTTG(+ACTCAC)GATCCTCACCCATTGCCTGGTGATAATGCTA NGG 10 8 17 8 24 0 100 5 10
    240604211816_lmdz CAGCCTAGCATTATCACCAGGCAATGGGTGAG(+ACTCAC)GATCCAAAAGAAAGTGCTGTTTTGTTATATT NGG 10 8 17 8 24 0 100 5 10
    240604211815_c0gq TGACAGGCAATTTCAACAGTTTAAAGTGTTTG(+ACTCAC)GTACCGTGGCTTCTGAGCGACCTGCAGTGGG NGG 10 8 17 8 24 0 100 5 10